Species Identification in Forensic Casework Analysis: Validation Studies on the Sequence Analysis of the Cytochrome B Gene. Walther Parson, Karin Pegoraro, Harald Niederstätter, Richard Scheithauer Institute of Legal Medicine, University of Innsbruck, Muellerstr. 44, Innsbruck, Austria 3⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄41⁄23⁄...

Background With some exceptions, mitochondria within the class Insecta have the same gene content, and generally, a similar gene order allowing the proposal of an ancestral gene order. The principal exceptions are several orders within the Hemipteroid assemblage including the order Thysanoptera, a sister group of the order Hemiptera. Within the Hemiptera, there are available a number of complet...

Mitochondrial DNA (mtDNA) is highly susceptible to mutations due to the low level of DNA repair and the presence of a high level of reactive oxygen species in the organelle. Although mtDNA mutations have been implicated in degenerating diseases, aging, and cancer, very little is known about the role of T cells in immunosurveillance for mtDNA aberrations. Here, we describe T-cell recognition of ...

The current study aimed to examine a real-time PCR assay with high-resolution melting (HRM) analysis for the species identification of minced meat samples. Meat samples from several animal were purchased and separately or as mixture two species. DNA was extracted all subjected by amplifying species-specific mitochondrial cytochrome b regions. Regarding mixtures, separate curves specific melt pe...

In the Xingu river basin, Brazil, occurs two genera of Potamotrygonidae family: Potamotrygon and Paratrygon. this region, taxa have significant economic importance for ornamental fishing industry, being intensively captured, especially species leopoldi, which is endemic to basin. attempt propose a species-specific DNA marker from Xingu, as well ensuring robust reliable molecular identification,...

Phlebotomus papatasi is an important vector of L. major in Iran. P. papatasi was collected from peridomestic animal shelters, inside and around the houses and also the nearby burrows of the gerbil reservoir hosts, Rhombomys opimus, in several provinces in Iran. Mitochondrial Cytochrome b (Cyt b) of sandflies, which is a maternally-inherited gene marker, was used to see if there is any "isolatio...

AIM Partial fragment of D-loop region extending from 35 to 770 were compared with corresponding sequences of 16 wild pigs and 9 domestic pig breeds from different parts of the world for detection of single nucleotide polymorphism (SNP) markers in the region. The paper also reappraises SNP markers from two fragments of cytochrome b gene and a fragment 12S rRNA gene distinguishing the Indian wild...

The recent demonstration that certain mitochondrial subunits of cytochrome oxidase (CO) are regulated by neuronal activity has stimulated interest in the molecular processes that coordinate nuclear and mitochondrial gene expression following synaptic stimulation. We have studied the constitutive expression and activity-guided regulation of cytochrome b (cyt b), a gene that is encoded by mitocho...

CONFLICT OF INTEREST none declared. INTRODUCTION This study was carried out to evaluate PCR-based method for detection of DNA in goat milk. It utilized primers targeting the mitochondrial cytochrome -b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. METHODS For the specific identification of goat mtcyt-b gene, pair of primers (GSL1, GSR2), were used, which produced a 4...