ABSTRACT At the onset of mitosis, centrosomes expand pericentriolar material (PCM) to maximize their microtubule-organizing activity. This step, termed centrosome maturation, ensures proper spindle organization and faithful chromosome segregation. However, as expands, how PCM proteins are recruited held together without membrane enclosure remains elusive. We found that endogenously expressed pe...

The reconstruction of the nucleolus after mitosis was analyzed by electron microscopy in cultured mammalian (L929) cells in which nucleolar RNA synthesis was inhibited for a 3 h period either after or before mitosis. When synchronized mitotic cells were plated into a concentration of actinomycin D sufficient to block nucleolar RNA synthesis preferentially, nucleoli were formed at telophase as u...

Mitochondria—critical metabolic hubs in eukaryotic cells—are involved a wide range of cellular functions, including differentiation, proliferation, and death. Mitochondria import most their proteins from the cytosol linear form, after which they are folded by mitochondrial chaperones. However, despite extensive research, extent to function particular chaperones is essential for maintaining spec...

Ubiquitin-mediated proteolysis is critical for the alternation between DNA replication and mitosis and for the key regulatory events in mitosis. The anaphase-promoting complex/cyclosome (APC/C) is a conserved ubiquitin ligase that has a fundamental role in regulating mitosis and the cell cycle in all eukaryotes. In vertebrate cells, early mitotic inhibitor 1 (Emi1) has been proposed as an impor...

Entry into and progression through mitosis depends on phosphorylation and dephosphorylation of key substrates. In yeast, the nucleolar phosphatase Cdc14 is pivotal for exit from mitosis counteracting Cdk1-dependent phosphorylations. Whether hCdc14B, the human homolog of yeast Cdc14, plays a similar function in mitosis is not yet known. Here we show that hCdc14B serves a critical role in regulat...

The reconstruction of the nucleolus after mitosis was analyzed by electron microscopy in cultured mammalian (L929) cells in which nucleolar RNA synthesis was inhibited for a 3 h period either after or before mitosis . When synchronized mitotic cells were plated into a concentration of actinomycin D sufficient to block nucleolar RNA synthesis preferentially, nucleoli were formed at telophase as ...

Cancer cells proliferate unlimitedly. Therefore, targeting the proliferative capacity of cancer is one of the smartest ways to treat cancer. In this vein, understanding the basis of mitosis is essential for the advanced treatment of cancer, let alone its importance in basic biology. Indeed, recent progress in anti-cancer strategy has come from the development of a group of inhibitors targeting ...

BACKGROUND Many organisms undergo closed mitosis and locate tubulin and mitotic kinases to nuclei only during mitosis. How this is regulated is unknown. Interestingly, the NIMA kinase of Aspergillus nidulans interacts with two nuclear pore complex (NPC) proteins and NIMA is required for mitotic localization of the Cdk1 kinase to nuclei. Therefore, we wished to define the mechanism by which the ...

Mitosis entails mechanistic changes required for maintaining the genomic integrity in all dividing cells. The process is intricate and temporally spatially regulated by ordered series of activation de-activation protein kinases. mitotic kinases ensure stepwise progression entry into mitosis after G2 phase cell cycle, followed prophase, pro-metaphase, metaphase, anaphase, telophase, subsequently...

Although of short duration, mitosis is a complex and dynamic multi-step process fundamental for development of organs including the brain. In the developing cerebral cortex, abnormal mitosis of neural progenitors can cause defects in brain size and function. Hence, there is a critical need for tools to understand the mechanisms of neural progenitor mitosis. Cortical development in rodents is an...