In contrast to mammalian matrix metalloproteinases (MMPs) that play important roles in the remodeling of the extracellular matrix in animals, the proteases responsible for dynamic modifications of the plant cell wall are largely unknown. A possible involvement of MMPs was addressed by cloning and functional characterization of Sl2-MMP and Sl3-MMP from tomato (Solanum lycopersicum). The two toma...

Mammalian kidney development is initiated by the mutual interaction between embryonic metanephric mesenchyme (MM) and the ureteric bud (UB), leading to tightly controlled UB branching morphogenesis. In a three-dimensional cell culture model, which employs MM cell-derived conditioned medium (BSN-CM) to induce UB cell branching morphogenesis in extracellular matrix (ECM) gels (Sakurai H, Barros E...

conclusions our results suggest that tetracyclines dose-dependently inhibit mmp-9 activity, and among the common clinical tetracyclines, minocycline provides the most potent inhibition of excess mmp-9 activity and its consequences. materials and methods an mmp-9-rich culture medium of u-937 cells was used as the source of the enzyme. serial dilutions of 5, 20, 100, 200, and 500 µm of each drug ...

Matrix metalloproteinases (MMPs) are a heterogeneous family of endopeptidases that play role in many physiological functions, including the immune response. An imbalance between activity MMPs and their tissue inhibitors (TIMPs) has been proposed pathophysiology different autoimmune disorders. We aimed to assess plasmatic levels MMP-2, MMP-9, TIMP-1 -2 patients with chronic inflammatory demyelin...

Objective and Impact Statement. There is a need to develop platforms delineating inflammatory biology of the distal human lung. We describe platform technology approach detect in situ enzyme activity observe drug inhibition lung using combination matrix metalloproteinase (MMP) optical reporters, fibered confocal fluorescence microscopy (FCFM), bespoke delivery device. Introduction . The develop...

The matrix metalloproteinases (MMPs) are implicated in the pathology of multiple sclerosis (MS). This review summarizes the consequences of upregulation of MMP members in MS as well as in an animal model of the disease, experimental autoimmune encephalomyelitis (EAE). The pathogenic roles of MMPs are considered, especially in the transmigration of leukocytes into the CNS. We review the evidence...

BACKGROUND AND OBJECTIVES Alcoholic fibrosis and its end stage cirrhosis represent a major health problem worldwide. Liver fibrosis occurs when the rate of matrix synthesis exceeds matrix degradation. The degree of matrix remodeling depends on the ratio of active matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). The objective of the present work was to study ...

The matrix metalloproteinase (MMP) family of extracellular proteinases play roles in normal physiological processes as well as in multiple disease settings including cancer. The link between MMP activity and cancer was considered strong enough to warrant considerable investment in pharmacological inhibitors of MMPs as a potential therapeutic modality, however, multiple large-scale clinical tria...

In chronic respiratory disease, matrix metalloproteinases (MMPs) contribute to pathological tissue destruction when expressed in excess, while tissue inhibitors of metalloproteinases (TIMPs) counteract MMPs with overexpression leading to fibrosis formation. They may be out of balance in equine pneumopathies and serve as biomarkers of pulmonary inflammation. We hypothesized that MMPs and TIMPs c...