BACKGROUND The accurate and rapid identification of the causative viruses is important for the timely diagnosis and management of respiratory infections. Multiplex molecular diagnostic techniques have been widely adopted to detect respiratory viruses. We compared the results of a newly upgraded, multiplex, molecular bead-based respiratory viral panel (RVP) assay with the results of Anyplex II R...

ABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, has often been linked to oral transmission through açai consumption. Molecular methods that allow fast and accurate identification of pathogen are important for detection presence parasite in this food. This study aimed optimize polymerase chain reaction (PCR)-based T. cruzi DNA pulp. Several dilutions DTU TcI trypomastigote fo...

Rational design for a new spiroxindoles, combined with benzimidazole scaffold to identify murine double minute two (MDM2) inhibitor was synthesized and characterized. The desired spiroxindoles were achieved via [3+2] cycloaddition reaction approach which afforded the cycloadducts four asymmetric centers separated in an excellent regioselective diastereoselective compound. subjected set of bioch...

Objective: The primary objective was to study the in vitro antioxidant and anticancer evaluation of novel 5-benzylidene substituted rhodanine derivatives molecular docking studies most active compounds with 3 different targets. Methods: Antioxidant potential were studied by DPPH assay, done MTT assay Computational using various softwares such as ACD Lab Chemsketch 12.0, molinspiration Discovery...

The ecological and health concern of mutagenicity and carcinogenicity potentially associated with an overwhelmingly large and ever-increasing number of chemicals demands for cost-effective and feasible method for genotoxicity screening and risk assessment. This study proposed a genotoxicity assay using GFP-tagged yeast reporter strains, covering 38 selected protein biomarkers indicative of all ...

Hfq is a bacterial master regulator which promotes the pairing of nucleic acids. Due to high molecular weight complexes formed between acids and amyloid form protein, it difficult analyze solely by gel shift assay formed, as they all migrate at same position in gel. In addition, precise kinetics measurements are not possible using assay. Here, we used synchrotron-based biophysical approach, syn...

Molecular detection of herpes simplex virus (HSV) DNA is recognized as the reference standard assay method for the sensitive and specific diagnosis of central nervous system infections caused by HSV. In this study, a molecular assay based on real-time PCR on the LightCycler (LC) instrument was evaluated and compared with a home-brew molecular assay. The detection limit of the LC assay was deter...

conclusions the in-house 1 step taqman real time rt-pcr assay showed acceptable performance characteristics. our study presents the robustness and cost-effectiveness of the method for detection and quantification of hcv rna. methods the primers and probe were selected from a highly conserved region of the hcv genome, which allowed the detection of 4 common hcv genotypes in iran. using 4 quantif...

BACKGROUND Campylobacter fetus is a pathogen of major concern for animal and human health. The species shows a great intraspecific variation, with three subspecies: C. fetus subsp. fetus, C. fetus subsp. venerealis, and C. fetus subsp. testudinum. Campylobacter fetus fetus affects a broad range of hosts and induces abortion in sheep and cows. Campylobacter fetus venerealis is restricted to catt...