Imaging mass spectrometry (IMS) is a powerful technique that enables analysis of various molecular species at high spatial resolution with low detection limits. In contrast to the matrix-assisted laser desorption/ionization (MALDI-MS) approach, surface-assisted (SALDI) can be more effective in small molecules due absence interfering background signals m/z ranges. We developed functionalized TiO...

Molecularly imprinted polymers (MIP) exhibiting high selectivity and affinity to the predetermined molecule (template) are now seeing a fast growing research. However, optimization of the imprinted products is difficult due to the fact that there are many variables to consider, some or all of which can potentially impact upon the chemical, morphological and molecular recognition properties of t...

This review describes recent molecular biological research on olfactory chemoreception in fishes. The recent rapid development of molecular biological techniques has provided new valuable information on the main and vomeronasal olfactory receptor (OR) genes, the axonal projection from ciliated, microvillous and crypt-olfactory receptor cells to the olfactory bulb, properties of odorant substanc...

Mechanisms of interaction of a three-dimensional polymer with solutes are of interest in the development and function of synthetic biomimetic polymers. We have investigated the effects of template/monomer interactions and template concentration on conversion and polymerization rates of a molecularly imprinted polymer. Copolymers of 2-hydroxyethyl methacrylate (HEMA) and poly(ethylene glycol) di...

Uniformity recognition sites for water-soluble ionic compounds were constructed onto the pores of porous polymer particles. This concept is based on a modified interval immobilization technique, which was used for the selective recognition of paralytic shellfish poison, saxitoxins (STXs). The results of batch adsorption and solid-phase extraction for one of the STX analogues, a prepared polymer...

Molecular imprinting is the differential expression and/or silencing of alleles according to their parent of origin [1, 2]. Conflicts between parents, or parents and offspring, should cause "arms races," with accelerated evolution of the genes involved in imprinting. This should be detectable in the evolution of imprinting genes' protein sequences and in the promoter regions of imprinted genes....

Converging evidence implicates the intermediate and medial mesopallium (IMM) of the domestic chick forebrain in memory for a visual imprinting stimulus. During and after imprinting training, neuronal responsiveness in the IMM to the familiar stimulus exhibits a distinct temporal profile, suggesting several memory phases. We discuss the temporal progression of learning-related biochemical change...

The new technique of molecular imprinting has increasingly been adopted by research laboratories worldwide during the last few years. We have studied the use of such imprints against drugs as artificial antibody-binding mimics in competitive radioimmuno-style binding assays. The recognition sites "molded" in the polymers mimic the binding sites of natural antibodies in their interactions with t...

The translation of genetic messages into proteins on ribosomes is catalyzed by a number of proteins that bind transiently to the ribosome [1,2]. These are the initiation, elongation and release (termination) factors. Several of these factors are GTP-hydrolyzing (G) proteins, which undergo a conformation transition between an active state with bound GTP and an inactive state with bound GDP. In b...