this study was conducted to optimize a highly efficient mrna transfection into dendritic cells (dc) derived from esophageal squamous cell carcinoma (escc) patients. applying an electroporation technique, in vitro synthesized green fluorescent protein (gfp) mrna was transfected as an indicator into the dcs derived from a healthy donor. flow cytometry revealed 84.9% transfection efficiency for dc...

Background: Maternal mRNA degradation is a selective process that occurs in waves corresponding to important developmental transitions such as resumption of meiosis, fertilization and zygotic genome activation. It has been demonstrated that the number, position, and combination of 3 UTR cis-acting elements interacting with trans-acting protein factors regulate translation and mRNA stability. Ou...

Protein synthesis is one of the most complex cellular processes, involving numerous translation components that interact in multiple sequential steps. The most complex stage in protein synthesis is the initiation process. The basal set of factors required for translation initiation has been determined, and biochemical, genetic, and structural studies are now beginning to reveal details of their...

A central question in understanding cytokinesis is how the cleavage plane is positioned. Although the positioning signal is likely to be transmitted via the anaphase microtubule array to the cell cortex, exactly how the microtubule array determines the site of contractile ring formation remains unresolved. By analysing tum/RacGAP50C mutant Drosophila embryos we show that cells lacking Tum do no...

Paraganglioma is a less prevalent disease, and paraganglioma with only secreting interleukin-6 (IL-6) has not been previously reported. A 64-year-old male patient came to the hospital chief complaints of fever palpitations. The peak body temperature was 38.7°C (101.66°F). Heart rate 110 bpm, while blood pressure in normal range. Antibiotics antiviral therapies were ineffective. levels IL-6, C-r...

Introduction: Reactive oxygen species (ROS) have been suggested to play an important role in the myocardial damage induced by ischemia – reperfusion. One element believed to be activated by ROS and to contribute to the reduction of ROS production, is the uncoupling protein-2 (UCP2). The objective of this investigation was to explore the effect of myocardial ischemia reperfusion on cardiac UC...

Swissmedic und die Europäische Arzneimittelagentur informierten, dass Peri-/Myokarditiden nach Applikation der Messenger-RNA-(mRNA-)COVID-19-Impfstoffe Tozinameran (Pfizer/­BioNtech) mRNA-1273 (Moderna) auftreten können.

The mRNA export complex TREX (TREX) is known to contain Aly, UAP56, Tex1 and the THO complex, among which UAP56 is required for TREX assembly. Here, we systematically investigated the role of each human TREX component in TREX assembly and its association with the mRNA. We found that Tex1 is essentially a subunit of the THO complex. Aly, THO and UAP56 are all required for assembly of TREX, in wh...