The corneas of New Zealand albino rabbits were isolated with a sclero-corneal rim for storage in a modified tissue culture medium (M-K medium) at 4° C. The corneas were either mounted in the specular microscope for daily observation of the endothelial layer and comeal thickness while being bathed in the storage medium at 4° C, or sealed in vials with the storage media. The vials were stored at ...

OBJECTIVE Urine is the most frequent specimen received for culture/sensitivity by clinical laboratories. The microbiological performance of HiCrome UTI agar medium was compared with Blood agar and MacConkey agar for isolation and presumptive identification of bacteria from urine culture. METHODS A total of 443 consecutively collected midstream and/or catheter-catch urine samples from patients...

Metallothionein is an ubiquitous metal-binding protein which is inducible by various stimuli such as Cd. Cu, Zn, dexamethasone or various "stress" factors. In rat liver, there m two isoforms of metallothionein (MT-I and MT-2) but i t is unknown if they have separate functions [ I ] . Antisense oligodeoxynucleotides (ODNs) are commonly used to reduce the synthesis of a protein in order to study ...

background: acanthamoeba-related disease have a poor prognosis according to many previous studies. thus researches regarding biochemical and molecular aspects of this organism are a high priority. to this end achieving high amount of amoebae in culture is the first step for such studies. the main aim of the present research was to address the usage of tyi-s-33 (tripticase, yeast extract, iron-s...

objective: in all protocols for isolation of mesenchymal stem cells (mscs), a few days after culture initiation, the medium were discarded along with its contents of non-adherent cells and the adherent cell population kept and expanded as mscs population. in the present study, attempt was made to expand the cells suspended in removed medium of primary culture and compare them with the adherent ...

the purpose of this research was to design a semi-industrial culture medium to obtain the maximum biomass production from bacterium bacillus subtilis utb96. in the first step, in order to reduce the cost of culture medium components, commercial products and industrial by products including, sugar beet molasses, sugar cane molasses, potato waste water and milk serum as carbon sources and corn st...

Metallothionein (MT) is an intracellular metal-binding, cysteine-rich protein, and is a potent antioxidant that protects cells and tissues from oxidative stress. Although the major isoforms MT-1 and -2 (MT-1/-2) are highly inducible in many tissues, the distribution and role of MT-1/-2 in diabetic nephropathy are poorly understood. In this study, diabetes was induced in adult male rats by strep...