1. Purified myelin labelled with [3H]myo-inositol or [1-14C]acetate was incubated with trypsin or acetylated trypsin at 37°C, pH 8.0 for 30min. 2. After incubation and centrifugation analysis of the myelin pellet showed marked digestion of basic protein on polyacrylamide-gel electrophoresis. Proteolipid and Wolfgram proteins remained unchanged. 3. A loss of 15% of total protein and loss of all ...
