UNLABELLED Owing to their small size and enhanced stability, nanobodies derived from camelids have previously been used for the construction of intracellular "nanotraps," which enable redirection and manipulation of green fluorescent protein (GFP)-tagged targets within living plant and animal cells. By taking advantage of intracellular compartmentalization in the magnetic bacterium Magnetospiri...

Single-domain antibodies, known as nanobodies, have great potential biorecognition elements for sensors because of their small size, affinity, specificity, and robustness. However, facile efficient methods nanobody immobilization are sought that retain maximum functionality. Herein, we describe the direct nanobodies on gold by exploiting a modified cysteine strategically positioned at C-termina...

BACKGROUND Over the last few decades the methylotrophic yeast Pichia pastoris has become a popular host for a wide range of products such as vaccines and therapeutic proteins. Several P. pastoris engineered strains and mutants have been developed to improve the performance of the expression system. Yield and quality of a recombinant product are important parameters to monitor during the host se...

BACKGROUND The thorough understanding of the physiological and pathological processes mediated by extracellular vesicles (EVs) is challenged by purification methods which are cumbersome, not reproducible, or insufficient to yield homogeneous material. Chromatography based on both ion-exchange and immune-capture can represent an effective method to improve EV purification and successive analysis...