Abstract Extraction of hazardous metals from dumped crystal glass waste was investigated for site decontamination and resource recovery. Mechanically activated leached with biodegradable chelating agents ethylenediamine- N , N′ -disuccinic acid (EDDS) nitrilotriacetic (NTA), where the concentration reaction time were determined by using Box–Wilson experimental design. Hazardous lead (Pb), arsen...

OBJECTIVE In this study, kinetic analysis was performed to understand the functional importance of the amino terminal of the active site of previously mutated Plasmodium vivax Lactate Dehydrogenase enzyme by mimicking Toxoplasma gondii I, II, Eimeria acervulina and Eimeria tenella LDH's. MATERIAL AND METHODS Mutant LDH genes were amplified by PCR and 6xHistag was added to the C-terminal of th...

When assessing the benefits and risks of transgenic crops, one consideration is their relative effects on non-target arthropod (NTA) abundance and functions within agroecosystems. Several laboratory and field trials have been conducted in Spain since the late 1990s to assess this issue. A consideration in the design of field trials is whether it is necessary to sample most NTAs living in the cr...

Ni-NTA functionalized iron oxide magnetic nanoparticle was synthesized and used to selectively immobilize a his-tagged enzyme from cell free extract as an active and recyclable nanobiocatalyst, where purification and immobilization of the target enzyme were accomplished in one pot.

This article refers to the paper "A novel method for isolation and recovery of ceramic nanoparticles and metal wear debris from serum lubricants at ultra-low wear rates" (Lal et al., 2016) [1] and describes the concentration and size distribution data of silicon nitride nanoparticles measured using nanoparticle tracking analysis (NTA). A NanoSight LM10 instrument was used to capture the video d...

IMAC [ 141 2.1. Pass solubilized material (about 45 ml) at a flow rate of 0.4 ml/min over Ni-NTA resin equilibrated with buffer A. 2.2. Wash Ni-NTA column at a flow rate of 0.4 ml/min with 120 ml of buffer A. 2.3. Elute N T R fusion protein at a flow rate of 0.2ml/min with 80ml of buffer B*. Combine peak fractions (1 3 ml) and add protease inhibitors. Save 1 ml for analysis. 2.4. Dilute remaini...

The actin filament severing protein cofilin-1 (CFL-1) is required for actin and P-type ATPase secretory pathway calcium ATPase (SPCA)-dependent sorting of secretory proteins at the trans-Golgi network (TGN). How these proteins interact and activate the pump to facilitate cargo sorting, however, is not known. We used purified proteins to assess interaction of the cytoplasmic domains of SPCA1 wit...

The analytical technique DGT (Diffusive Gradients in Thin-films) is able to gain access a wealth of information by carefully interpreting accumulation data from passive samplers with different configurations (i.e. thicknesses its constituent layers). A set devices were simultaneously deployed solutions Ni and nitrilotriacetic acid (NTA) concentrations measure the availability these solutions. A...