We have examined nutritional factors influencing conjugal transfer of the two nonsymbiotic large plasmids, pRmeGR4a and pRmeGR4b, of Rhizobium meliloti GR4. To monitor transfer, each plasmid was tagged with a different antibiotic resistance marker. Transfer of plasmid pRmeGR4b was dependent upon the presence of plasmid pRmeGR4a on the same donor cell. Transconjugants for pRmeGR4b were obtained ...

The solubility of nitrogen in liquid silicon equilibrated with silicon nitride and its dependence on the composition of the atmosphere has been studied in the temperature region 1428–1542 C. High purity silicon was melted in silicon nitride crucibles under Ar and N2 atmospheres. The equilibrium was observed to be established within minutes, after which no evolution of the nitrogen content with ...

SnO2/nitrogen-doped graphene nanohybrids have been synthesized by an in situ hydrothermal method, during which the formation of SnO2 nanocrystals and nitrogen doping of graphene occur simultaneously. The as-prepared SnO2/nitrogen-doped graphene nanohybrids exhibit enhanced electrochemical performance for sodium-ion batteries compared to SnO2/graphene nanocomposites. A systematic comparison betw...

background : the study examined the implementation of artificial neural network (ann) for the prediction of ammonia nitrogen removal from landfill leachate by ultrasonic process. methods : a three-layer backpropagation neural network was optimized to predict ammonia nitrogen removal from landfill leachate by ultrasonic process. considering the smallest mean square error (mse), the configuration...

The significance of molecules containing difluoromethyl groups is driven by their potential applications in pharmaceutical and agrochemical science. Methods for the incorporation lightly fluorinated such as CF2H have been less well developed. Here we report use difluorinated diazoacetone a practical reagent direct synthesis CF2H-substituted 2-amidofurans through addition to ynamides. These newl...

Ultramicro methods for the determination of serum urea nitrogen, uric acid, and creatinine have been evaluated and the results are re corded in reports VII, VIII, and IX, respectively. The method for the determination of urea nitrogen is an ultramicro adaptation of the reaction in which a blue color is developed when the ammonium ion reacts with phenol and hypochlorite. Ammonia is released from...

Controlling nitrogen oxide (NOX) emissions is still a challenge as increasingly stringent emission limits are introduced. Strict regulations will lead to the need introduce secondary measures even for boilers with bubbling fluidized bed (BFB), which generally characterized by low NOX emissions. Selective non-catalytic reduction has lower investment costs compared other reduction, but temperatur...

We investigated the feasibility of gene transfer into the mouse central nervous system (CNS) by applying nanosecond pulsed laser-induced stress waves (LISWs). Intraventricular or hippocampal injection of a reporter gene [enhanced green fluorescent protein (EGFP)] followed by application of LISWs showed this method to be efficient in the CNS of newborn and adult mice. Cells expressing EGFP resid...