a modified pulsed-field gel electrophoresis (pfge) protocol was developed and applied to clinical isolates of mycobacterium tuberculosis complex to reduce the cost of using lyticase. this protocol reduces the expense of pfge typing of mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.

Rabbits were sensitized with killed Mycobacterium tuberculosis, M. kansasii, M. avium, M. scrofulaceum, M. fortuitum, and Nocardia asteroides, and their response to homologous and heterologous antigens was assessed in vitro by direct and indirect macrophage migration inhibition tests. The antigens were obtained by disintegration of bacterial mass and by purification of the supernatants by ultra...

Dear editor,I read with interest article that published entitled *Isolation and identification of bioactive compound producing Rhodococcus spp. isolated from soil samples {IJMCM/5(1) (2015) 463-468}* [1]. Some of the genus such as Nocardia, Gordonia, Mycobacterium and Rhodococcus are in actinomycete family and they are Gram-positive and partially acid-fast. Rhodococcus species usually stain Gra...

background and objectives: diagnosis of avian tuberculosis by conventional culture method is still considered as the “gold standard” technique. the main objective of this study was to compare growth of mycobacterium avium subsp. avium on four specific mycobacterial cultures such as glycerinated lowenstein-jensen medium, pyruvate-enriched lowenstein- jensen medium, mycobactin j-supplemented herr...

conclusions review of the studies revealed that levels of multidrug-resistant tuberculosis among new tuberculosis patients are increasing in iran and the vaccination system needs to be reformed. because of the increasing number of patients coming from neighboring countries with multidrug-resistant tuberculosis, this area needs more stewardship and control in order to prevent the outbreak of mul...

isoniazid, is the only antituberculous drug for which the relation between lack of virulence and acquisition of resistance was associated. inh-resistant mutants were shown to contain defective katg gene. classical studies showed that inh-resistant south indian isolates have lower virulence in guinea pigs and higher susceptibility to h2o2. it is of interest to assess the virulence in south india...

background: tuberculosis (tb) is a major cause of death worldwide. finding an effective vaccine against tb is the best way to control it. several vaccines against this disease have been developed but none are completely protective. the aim of this study was to design and construct a cloning vector containing the mycobacterium tuberculosis (m. tuberculosis) heat shock protein x (hspx). methods: ...

in most countries, tuberculosis caused by mycobacterium bovis is mainly a disease of cattle but can infect buffalos too. the disease can be controlled successfully by mean of a test-and-slaughter program. in iran test-and-slaughter program has started since 1971 and prevalence of bovine tuberculosis reduces from 5% to less than 0.12% in recent years. in western azarbaijan, north west of iran, t...

tuberculosis is a chronic, specifi c granulomatous disease and a major cause of death in developing countries. we report a case of tuberculosis presenting fi rst as periodontal loss of tooth support leading to loose teeth and gingival enlargement affecting a 17-year-old female patient without any pulmonary lesion. diagnosis was based on histopathological examination and positive adenosine deami...