Phosphate solublization activity of Bacillus subtilis in different carbon and nitrogen sources with insoluble form of phosphate sources like tricalcium phosphate and rock phosphate were studied. TCP solubilization with different carbon sources irrespective of incubation period can be related in the following order: glucose, fructose, arabinose, mannose, sucrose, lactose, and sorbitol. The pH dr...

Fluorescence in situ hybridization (FISH) is a useful method of determining the replication timing of specific genomic loci in mammals and of delineating replicon structures on DNA fibers in combination with in vivo replication labeling. In the case of simultaneous detection of a FISH probe and replicated forks, however, the DNA fibers are damaged by the DNA denaturation step for FISH detection...

The influence of sodium hydroxide addition during the thermo-chemical pretreatment of a microbial biomass has been evaluated in terms of particulate chemical oxygen demand (COD) solubilization and anaerobic biodegradability. Both COD solubilization and total solid elimination rates increased with the dose of NaOH added: COD solubilization reached 63%, and total solid elimination was 33% when 5 ...

Solubilization of integral membrane proteins is a process in which the proteins and lipids that are held together in native membranes are suitably dissociated in a buffered detergent solution. The controlled dissociation of the membrane results in formation of small protein and lipid clusters that remain dissolved in the aqueous solution. Effective solubilization and purification of membrane pr...

This study aimed to analyze and quantify the effect of different ratios vermicompost, phosphate rock, sulfur on P solubilization release by Pseudomonas fluorescens Ur21, identify optimal levels those variables for an efficient biofertilizer. Twenty experiments were defined surface response methodology based a central composite design (CCD), effects various quantities (encoded −1, 0, or +1) was ...

The DRB3 gene is a highly polymorphic major histocompatibility complex (MHC) class II gene and plays an important role in variability of immune responsiveness and disease resistance. In the present study, the MHC class II DRB3 gene in water buffalo (Bubalus bubalis) populations from Northwest regions of Iran was investigated through PCR-SSCP. Genomic DNA was extracted from whole blood samples c...

INTRODUCTION Functional alterations of the cystic fibrosis transmembrane conductance regulator gene (CFTR) increase the viscoelasticity of pulmonary secretions of cystic fibrosis (CF) patients and require the use of therapeutic aerosols. The biochemical properties of exocrine secretions are influenced by the expression of the FUT2 gene which determine the secretor and non-secretor phenotypes of...

The effects of temperature (25-40°C), H,SO, concentration (31-70% (w/v)) and the acid/substrate relationship (1-5 cm3 of H,SO, per g-' of cellulose) on the solubilization rate of microcrystalline cellulose and on the glucose production rate have been analysed. The solubilization process was by determining reducing groups present in solution. For acid/substrate relationships of more than 1 cm3 g...

BACKGROUND AND AIMS Iron is stored in hepatocytes in the form of ferritin and haemosiderin. There is a marked increase in iron rich haemosiderin in iron overloaded livers, and ferric iron in amounts exceeding the ferritin and haemosiderin binding capacity may promote free radical generation, causing cellular damage. The aim of this study was to characterise hepatic haemosiderin using four antib...