With the decreasing cost and availability of many newly developed bioinformatics pipelines, next-generation sequencing (NGS) has revolutionized plant systematics in recent years. Genome skimming been widely used to obtain high-copy fractions genomes, including plastomes, mitochondrial DNA (mtDNA), nuclear ribosomal (nrDNA). In this study, through simulations, we evaluated optimal (minimum) dept...

Basidiomata of a hitherto undescribed Limacella species were collected from the grasslands Punjab, Pakistan. We describe it as subtropicana, which is characterized by its viscid, white to creamy pileus, stipe, changing brown when bruised, subglobose basidiospores measuring 4.0?6.3 × 3.7?5.5 ?m, an ixotrichodermal pileipellis with subcylindric clavate terminal elements and common presence clamp ...

A phylogenetic study of the Opopanax and Crenosciadium was conducted to elucidate their circumscription infrageneric relationships. Sequence data from nrDNA ITS, cpDNA matK trnL-F were used determine relationship. Polymerase Chain Reaction (PCR) amplifications all regions performed using universal primers. Maximum Likelihood (ML) trees constructed PAUP* software, another tree ITS sequences show...

The reconstruction of reticulate evolutionary histories in plants is still a major methodological challenge. Sequences of the ITS nrDNA are a popular marker to analyze hybrid relationships, but variation of this multicopy spacer region is affected by concerted evolution, high intraindividual polymorphism, and shifts in mode of reproduction. The relevance of changes in secondary structure is sti...