In the present investigation, E/S antigen from O. columbianum was isolated by in vitro culture method and hyper immune serum was raised against this antigen in rabbits. On SDS – PAGE analysis, characterization, the E/S antigen revealed six polypeptides at 29.0, 38.0, 49.0, 71.0, 84.0 and 95.0 kDa. Further, three immuno-reactive polypeptides at 29.0, 38.0 and 49.0 kDa were detected in western bl...
