nique reported by Paty et al. (2). The procedure is as follows: Concentrate 3 mL of CSF 80-fold in a Minicon CS 15 spinal fluid concentrator (Amicon Corp., Lexington, MA 02173). Apply concentrated CSF equivalent to 12 zg of IgG to a Titan III cellulose acetate plate (Helena Labs., Beaumont, TX 77704), and electrophorese for 45 mm at a constant voltage of 180 V, using high-resolution sodium barb...

Cerebrospinal fluid (CSF) and serum samples from eight patients with bacteriologically proven (6) or clinically suspected (2) tuberculous meningitis were tested for the presence of anti-mycobacterial IgG antibodies by an affinity-mediated immunoblot technique. This technique is based on agarose gel isoelectric focusing of paired CSF and serum samples diluted to the same IgG concentration, and t...

Immunoglobulin has been measured and studied electrophoretically in cerebrospinal fluid (CSF) from 14 patients with Alzheimer's disease and 25 undemented controls. Presence or absence of the diagnosis of Alzheimer's disease was confirmed histologically, as these were postmortem specimens. There was no increased incidence of oligoclonal IgG bands in either group, and no significant differences i...

Clinical features of 18 patients with either lymphocytic meningoradiculitis (n = 17) or chronic encephalomyelitis (n = 1) due to Borrelia burgdorferi are reported. Arthropod bites were recorded in only seven patients. High titres of anti B burgdorferi antibodies were detected in sera from all patients and in CSF from 12 out of 17 patients tested. All patients had CSF abnormalities especially pl...

• antibodies to C. pneumoniae in the cerebrospinal fluid of patients with the disease [(1.) Yao, S., Stratton, C.W., Mitchell, W.M., Sriram, S. (2001). CSF oligoclonal bands in multiple sclerosis represent antibodies against Chlamydophila. Neurology 56, 1168-76. (2.) Fainardi, E., Castellazzi, M., Casetta, I. et al. (2004). Intrathecal production of Chlamydia pneumoniaespecific high-affinity an...

Two methods of electrophoresis for the detection of oligoclonal bands in unconcentrated CSF were compared. A sample of 98 routine CSFs yielded 18 positives by polyacrylamide gel electrophoresis (PAGE) while cellulose acetate electrophoresis with immunofixation (CAIF) gave 13 positives (72% of the PAGE findings). Despite the loss of sensitivity the cellulose acetate electrophoresis was easier to...

• antibodies to C. pneumoniae in the cerebrospinal fluid of patients with the disease [(1.) Yao, S., Stratton, C.W., Mitchell, W.M., Sriram, S. (2001). CSF oligoclonal bands in multiple sclerosis represent antibodies against Chlamydophila. Neurology 56, 1168-76. (2.) Fainardi, E., Castellazzi, M., Casetta, I. et al. (2004). Intrathecal production of Chlamydia pneumoniae-specific high-affinity a...

tients. Clin Chem 1995;41:256–63. 9. Niederwieser G, Bonelli RM, Brunner D, Neubauer M, Buchinger W, Reisecker F, et al. Diagnostic accuracy of an agarose gel electrophoretic method in multiple sclerosis. Clin Chem 2001;47:144. 10. Cavuoti D, Baskin L, Jialal I. Detection of oligoclonal bands in cerebrospinal fluid by immunofixation electrophoresis. Am J Clin Pathol 1998;109:585–8. 11. Richard ...