Background and Aims: Finding prevalence of chicken anemia virus (CAV) infection in native chickens is necessary to avoid transmission of infection to commercial flocks. In this paper we attempt to describe molecular detection of chicken anemia virus in native Larry-breed chickens in Chaharmahal-va-Bakhtiyari province in Iran for the first time. Materials and Methods: Blood samples were collect...

The 65 serotypes of human enteroviruses are classified into four species, Human enterovirus (HEV) A to D, based largely on phylogenetic relationships in multiple genome regions. The 3'-non-translated region of enteroviruses is highly conserved within a species but highly divergent between species. From this information, species-specific RT-PCR primers were developed that can be used to rapidly ...

This study was conducted to determine the electrophoretic pattern of serum protein in goats infected withBabesia ovis. Serum total protein was calculated and serum electrophoresis from 15 goats naturally infectedwith B. ovis as well as same number of healthy goats was performed. Microscopic examination of Giemsastained peripheral blood smears revealed B. ovis infection. The parasitological diag...

Monilinia laxa is a pathogen of brown rot of stone fruit and almond in California, causing blossom blights and fruit rots. In this study, low-level resistance to the benzimidazole fungicides benomyl and thiophanate-methyl was detected in field isolates of M laxa collected from stone fruits and almonds in California. Low-resistant (LR) isolates grew in potato dextrose agar (PDA) plates amended w...

From complete mitochondrial DNA sequence of Fasciola hepatica available in Genbank, specific primers were designed for a conserved and repetitive region of this trematode. A pair of primers was used for diagnosis of infected Lymnaea columella by F. hepatica during the pre-patent period simultaneously with another pair of primers which amplified the internal transcribed spacer (ITS) region of rD...

1 CIRAD, Etiologie dépérissement, UPR 29, Campus international de Baillarguet, 34398 Montpellier cedex 5, France 2 OPRI-CIRAD Laboratory, Coconut Programme, P.O. Box 245, Sekondi, Ghana Abstract: Coconut palms (Cocos nucifera L.) can be affected by several types of Lethal Yellowing (LY) diseases worldwide. Some of the syndromes are caused by phytoplasmas, small bacteria...

The main objective of this study was to develop a reliable detection method for genetically modified cotton events MON 531 and MON 15985 by using multiplex PCR reaction technique. In this study we have used four primer pairs for the detection of individual gene segments present in transgene cassette of two GM cotton events which include CaMv35S promoter, Nos terminator, Cry1Ac and Npt II genes ...

CONFLICT OF INTEREST none declared. INTRODUCTION This study was carried out to evaluate PCR-based method for detection of DNA in goat milk. It utilized primers targeting the mitochondrial cytochrome -b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. METHODS For the specific identification of goat mtcyt-b gene, pair of primers (GSL1, GSR2), were used, which produced a 4...

Nuclear molecular evidence, for example, the rapidly evolving Internal Transcribed Spacer region (ITS), integrated with maternally inherited (mitochondrial) COI barcodes, has provided new insights into the diversity of clitellate annelids. PCR amplification and sequencing of ITS, however, are often hampered by poor specificity of primers used. Therefore, new clitellate-specific primers for ampl...

The aim of this study was to evaluate the genetic diversity sour cherries using SSR markers, correlate data with phenotypic traits, and investigate suitability Prunus-specific microsatellite markers in species. Nineteen cherry genotypes from Fruit Research Institute Érd, Hungary, were analyzed twelve primer pairs. number alleles ranged two ten, a mean value 4.67 per locus. highest generated BPP...