A purified recombinant protein from Eimeria acervulina (3-1E) was used to vaccinate chickens in ovo against coccidiosis both alone and in combination with expression plasmids encoding the interleukin 1 (IL-1), IL-2, IL-6, IL-8, IL-15, IL-16, IL-17, IL-18, or gamma interferon (IFN-gamma) gene. When used alone, vaccination with 100 or 500 mug of 3-1E resulted in significantly decreased oocyst she...

Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 10, No. 12, December 2004 2257 species can cause disease (symptomatic infection) and death. C. felis and C. meleagridis infections showed low oocyst shedding (all seven patients had low to moderate oocyst loads in samples). On the contrary, C. parvum produced similar clinical manifestations but showed higher oocyst shedding; 46% had high to ...

A method for determining the infectious reservoir of malaria (K) and vector survival rate (P) by measuring oocyst size and discriminating between the most recent and other infections is described. In the laboratory the mean diameter of 3 d oocysts in Anopheles gambiae, kept at 26 degrees C, was 11.5 microns and the mean diameter at day 5 was 24.5 microns. Oocyst sizes in wild caught mosquitoes ...

Effective removal of Cryptosporidium parvum oocysts by granular filtration requires the knowledge of oocyst transport and deposition mechanisms, which can be obtained based on real time microscopic observation of oocyst transport in porous media. Attachment of oocysts to silica surface in a radial stagnation point flow cell and in a micromodel, which has 2-dimensional (2-D) microscopic pore str...

Between 1985 and 1987, fecal samples were collected from 71 bats representing 14 species (Desmodontidae, Molossidae, Noctilionidae, Phyllostomidae, Vespertilionidae) from 8 localities in 3 states (Beni, Pando, Santa Cruz) in Bolivia, South America. Of these, 2 black myotid bats (Vespertilionidae), Myotis nigricans, and 1 tent-making bat (Phyllostomidae), Uroderma magnirostrum, had oocysts in th...

Concurrent with recent advances seen with Cryptosporidium parvum detection in both treated and untreated water is the need to properly evaluate these advances. A micromanipulation method by which known numbers of C. parvum oocysts, even a single oocyst, can be delivered to a test matrix for detection sensitivity is presented. Using newly developed nested PCR-restriction fragment length polymorp...

The paper by Jenkins et al. (6) provides interesting data on Cryptosporidium parvum oocyst permeability and survival. The vital dye assay (4) relies upon oocyst permeability and exhibits limitations, especially in assessment of disinfectant efficacy (3). However, it is rewarding to note that it is considered to have utility and can provide pertinent data. Although we agree with many points addr...

coccidiosis is a common disease of poultry breeding industry in the world that cause by eimeria spp. objective of the present study was comparison of anticoccidial effect of diclazuril and toltrazuril and their effects on the breeding function and intestinal oocyst shedding rate. therefore, 300 broilers from ross breed with 47 gr weigth average were divided into 3 groups randomely. then chicken...

The paper by Jenkins et al. (6) provides interesting data on Cryptosporidium parvum oocyst permeability and survival. The vital dye assay (4) relies upon oocyst permeability and exhibits limitations, especially in assessment of disinfectant efficacy (3). However, it is rewarding to note that it is considered to have utility and can provide pertinent data. Although we agree with many points addr...