We have previously demonstrated that short oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs) exert a positive effect on weight loss and oocyst shedding associated with Eimeria infection when injected in vivo. The present work investigated the effects of in ovo vaccination with CpG ODNs and an Eimeria recombinant microneme protein (MIC2), alone or in combination, on susceptibil...

The amount of indole-3-acetic acid (IAA) was measured in peach fruits by gas chromatography-mass spectrometry-selective ion monitoring using an isotope dilution assay with [(13)C(6)]IAA as an internal standard throughout the growing season. Ethylene evolution of the fruit was also measured. IAA levels were 25 nanograms per gram fresh weight, 18 days after anthesis. Both IAA levels and rates of ...

Our program proposes to concentrate on the rapid recovery of signature biomarkers based on automated high-pressure, high-temperature solvent extraction (ASE) and/or supercritical fluid extraction (SFE) to produce lipids, nucleic acids and proteins sequentially concentrated and purified in minutes with yields especially from microeukaryotes, Gram-positive bacteria and spores. Lipids are extracte...

Fur animal farming was initiated during the 1890s on Prince Edward Island in Canada. Farmed silver foxes descend from animals caught from the wild on the island. Finnish fur farming increased during the post war period and in mid-1980s, there were about 6000 fur animal farms, mostly located in Southern Ostrobothnia (Fig.), producing about 8 million fur animals yearly. Currently, there are appro...

Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...

  The efficacy of Clindamycin and Monensin were evaluated in the prevention of oocyst shedding of kittens with Toxoplasma gondii (Tehran strain). In this study, 28 healthy kittens aged 1.5 – 2 months old divided randomly into 4 groups of seven. In group 1, Kittens were fed with the infected brain tissues of mice, all of seven kittens (100 %), shed oocyst, nearly 1 week after infection, which la...

Cryptosporidium oocysts were found in 43 out of 77 calves from two farms in Iwate Prefecture and nine farms on Tanegashima Island, Kagoshima Prefecture, Japan. The DNA fragments of 18S ribosomal RNA (18S rRNA) gene were amplified by a nested PCR from 43 oocyst-positive as well as one oocyst-negative samples. All of them were precisely identified as C. parvum by analyzing the nucleotide sequence...

A series of experiments were performed in an attempt to extract genomic DNA from a small number of Eimerian oocysts. Sonication, ammonia, ethanol and lysozyme were all found to be unsuitable for the digestion of Eimeria oocysts. The chemicals and enzyme given were not capable of either disruption or digestion of oocysts for nucleic acid extraction. They had the capability of penetrating the ooc...

the effects of nitrogen (n) and phosphate (p) concentrations and temperature on crude oil biodegradation by two bacterial strains pg01 and pg02, previously isolated from persian gulf, were assessed. in order to determine the optimum concentration of n and p sources and temperature for oil consumption by the isolates, bacteria were cultured in 100 ml agitating culture flasks containing mineral s...