background: in general, 15% of oocytes collected in art cycles are immature. these oocytes may be cryopreserved further for use in in-vitro maturation (ivm) program. objective: the aim of this study was to determine maturation capacity, morphometric parameters and morphology of human immature oocytes in both fresh ivm (fivm) and vitrified-ivm (vivm) oocytes. materials and methods: 93 women who ...

Background: A mouse model of lipopolysaccharide (LPS)-induced inflammation was used to investigate the effect of pharmacological inhibition of nuclear enzyme PARP-1 on oocyte maturation, apoptotic and necrotic death, as well as DNA integrity of follicular cells. Also, the relative expression of cumulus genes (HAS2, COX2, and GREM1) associated with oocyte developmental competence was assessed. M...

If ovarian stimulation is performed accordingly to new strategies available, the occurrence of ovarian hyperstimulation syndrome will be eradicated. The strategy is to stimulate all women with GnRH antagonist and in case of need to -induce final egg maturation with GnRH agonist.

BACKGROUND Infertility due to accelerated loss of ovarian follicles/oocytes may occur through numerous mechanisms. As a result, posthumous human oocyte donation, banking and maturation protocols for research and fertility restoration are current interests in reproductive medicine. METHODS A computer-generated sample of Utah residents (n = 704) were surveyed regarding demographics, willingness...

The in vivo function and phenotype of ovarian follicle cells are determined by many factors. When these cells are removed from the in vivo microenvironment and grown in a 2D in vitro environment, the function of the follicular cells is difficult to preserve. A collagen hydrogel was used to examine the hormone and oocyte maturation of ovary follicles in a 3D culture system. Ovarian follicles fro...

Reproductive efficiency in dairy cattle has been decreased over the last 5 decades despite significant gains in genetic selection, improved reproductive technologies and nutritional care. In high-yielding dairy cattle, the negative energy balance (NEB) during the first week post-partum adversely affects ovarian activity, mainly follicle growth and steroidogenesis. Circulating levels of non-este...

The aim of this study was to evaluate the effects of ovarian tissue vitrification and two-step in vitro culture on the metaphase II (MII) oocyte reactive oxygen species (ROS) level, mitochondrial transcription factor A (TFAM) expression and succinate dehydrogenase (SDH) activity. After collection of neonatal mouse ovaries, 45 ovaries were vitrified and the others (n = 45) were...

Objective In vitro maturation (IVM) is an artificial reproductive technology in which immature oocytes are harvested from the ovaries and subsequently matured in vitro. IVM does not require ovarian hyperstimulation and thus the risk of ovarian hyperstimulation syndrome (OHSS) is avoided. In this multicenter prospective cohort study we assessed the live birth rate per initiated in vitro maturati...

In the present study, we aimed at characterizing the effect of prochloraz, an imidazole fungicide, on the oocyte meiotic maturation process in a freshwater teleost species, the rainbow trout (Oncorhynchus mykiss). Full-grown post-vitellogenic ovarian follicles were incubated in vitro with prochloraz, Luteinizing Hormone (LH), or a combination of prochloraz and LH. The occurrence of oocyte matur...