OBJECTIVE This study aimed to evaluate the expression of the genes related to folliculogenesis after vitrification of mouse ovarian tissues using a two-step in vitro culture. MATERIALS AND METHODS In this experimental study, vitrified and non-vitrified ovaries from 7- day old (neonate) female mice were cultured using alpha-Minimum Essential Medium (α-MEM) supplemented with 5% fetal bovine ser...

In vitro culture of human ovarian tissue the following cryopreservation is proposed for follicular development. There are no techniques that guarantee successful maturation of the follicles within the excised tissue. The viability of cultured human ovarian tissue improved by adding some growth factors to the culture media. The efficiency of vitrification as the cryopreservation method for human...

Background Ovarian tissue cryopreservation is an option for patients who require immediate gonagonadotoxic treatment of cancers. when there is insufficient time to conduct ovulation induction This procedure can be used in patients with genetic mutations with a high risk for premature ovarian failure.This strategy is the only option for fertility preservation in prepubertal girls or in women who...

Controlled ovarian hyperstimulation (COH) affects adversely implantation following in vitro fertilization and embryo transfer. COH with gonadotropins alters endometrial receptivity and luteal function. In addition periovulatory endometrial characteristics in stimulated cycles are considerably different compared with the natural cycles, and periovulatory secretory transformation is consistently ...

Ovarian hyperstimulation syndrome (OHSS) still remains a life-threatening complication of in vitro fertilization treatment (IVF), keeping patients and especially those, who previously experienced OHSS, from attempting infertility treatment and childbearing. The recent implementation of four new modalities: the GnRH antagonist protocol, GnRH agonist (GnRHa) triggering of ovulation, blastocyst tr...

Slow freezing has been a standardmethod for ovarian tissue cryopreservation and transplantation (OTCP), while vitrification is commonly used for cryopreservation of embryos, oocytes and sperm. We have conducted preclinical studies using cynomolgus monkeys since 2006 (Hashimoto et al., 2010; Suzuki et al., 2012) and clinical studies since 2010, reporting a live birth after OTCP with vitrificatio...

Background: Cryopreservation of ovarian tissue is an important option in order to preserve the fertility of cancer patients undergoing chemotherapy and radiotherapy. There are many reports on adding some materials such as calcium, ascorbate and antioxidants to freezing medium. Vanadium is one of the minerals, which is very important as a trace element for normal cell function as well as develop...

Background There are several indications of human female gamete cryostorage including sub-fertile and fertile patients. But our focus will be in women at risk of losing their reproductive function caused by oncologycal treatments or premature ovarian failure that could benefit greatly from this practice. Fertile women may take advantage of this technology to electively delay childbearing. The o...

BACKGROUND Successful cryopreservation of oocytes and embryos is essential not only to maximize the safety and efficacy of ovarian stimulation cycles in an IVF treatment, but also to enable fertility preservation. Two cryopreservation methods are routinely used: slow-freezing or vitrification. Slow-freezing allows for freezing to occur at a sufficiently slow rate to permit adequate cellular deh...