PURPOSE Neurochemical changes in nerve cells were investigated in the lateral geniculate nucleus (LGN) and primary visual cortex of macaque monkeys with experimentally induced glaucoma. METHODS Glaucomatous damage was induced in one eye of experimental animals by elevation of intraocular pressure following laser burns to the trabecular meshwork. Staining for the metabolic marker cytochrome ox...

In the management of bacterial infection, identification of the pathogen after growth remains essential, so that the most appropriate treatment can be proposed even before antibiotic susceptibility data are available. The strategy for most common pathogens requires Gram staining, simple tests (eg, determination of oxidase and catalase activity), and appropriate phenotypic tests that require com...

Neisseria gonorrhoeae was cultivated in a human diploid cell strain (WI-38). Eighty percent of the cultures contained viable gonococci for at least 4 m at 36°C, as evidenced by subculture to brain heart infusion broth. Monthly subcultures of bacteria could be made to fresh WI-38 cultures for at least 11 monthly passages with a 69% survival rate. The identity of gonococci was confirmed by morpho...

A laboratory is a place or room carrying out research, learning, and experiment activities equipped with various equipment materials supporting the work. This study aims to identify bacteria on nutrient agar plates at Universitas Muhammadiyah Palangkaraya’s Microbiology Laboratory. uses observational research non-probability sampling technique cross-sectional design. Bacterial samples were take...

The importance of oxidase-positive, nonfermentative, Gram-negative bacilli in a variety of pathological processes is becoming increasingly important. Oligella urethralis is an organism which is normally isolated as a commensal from the genitourinary tract. We have described one case of urinary tract infection caused by this organism. This isolated organism is found to be resistant to quinolones...

In 12 of 20 (60%) biopsy-proven cases of Pneumocystis carinii pneumonia, the diagnosis was first suggested by examination of routine Gram stains of impression smears made from infected lung tissue and later confirmed by methenamine-silver staining. The cysts appeared as 5- to 7-microns unstained spheres, each containing six to eight intracystic gram-negative bodies (sporozoites). Although the G...

A new staining procedure for glucose oxidase at both the light and the electron microscopic level has been developed. The procedure was equally effective for the detection of galactose oxidase. It appears that the method can be applied to all oxidoreductases which catalyze the production of hydrogen peroxide. Aspergillus niger glucose oxidase and Dactylium dendroides galactose oxidase were used...

It has been shown previously (Kennedy and Barbaro, 1953) that the amount of crystal violet adsorbed by bacteria can be measured quantitatively by either one of two procedures. One procedure involved micro-Kjeldahl analyses of bacterial cells before and after staining. The increase in total nitrogen in the stained cells represented the dye nitrogen adsorbed. The second procedure was based on mic...