This unit describes how to use the genome annotation and curation tools MAKER and MAKER-P to annotate protein-coding and noncoding RNA genes in newly assembled genomes, update/combine legacy annotations in light of new evidence, add quality metrics to annotations from other pipelines, and map existing annotations to a new assembly. MAKER and MAKER-P can rapidly annotate genomes of any size, and...

“The pituitary has an intrinsic rhythm of small amplitude with a frequency of every two to ten minutes. Superimposed on this intrinsic rhythm is a rhythm caused by the pulsatile release of hypophysiotropic releasing factors, with or without the withdrawal of a corresponding inhibitory factor. Rhythms that are shorter than a day are referred to as ultradian rhythms. The next layer of rhythmicity...

The zebrafish pineal gland (epiphysis) is a site of melatonin production, contains photoreceptor cells, and functions as a circadian clock pace maker. Here, we have used microarray technology to study the zebrafish pineal transcriptome. Analysis of gene expression at three larval and two adult stages revealed a highly dynamic transcriptional profile, revealing many genes that are highly express...

The World Wide Web (WWW) can be viewed as the largest multime-dia database that has ever existed. However, its support for query answering and automated inference is very limited. Metadata and domain speciic ontologies were proposed by several authors to solve this problem. We developed Ontobroker which uses formal ontologies to extract, reason, and generate metadata in the WWW. The paper descr...

Regular uterine contractions in normal labor are expulsive power to deliver the fetus. The intrauterine pressure changes were recorded and studied in the measurement of labor contractions of uterus, while external tocodynamometry is common in clinical fetal monitoring [1]. The duration of contraction was about 1 min and interval was also 1 min, and the peak intrauterine pressure was 30 to 50 mm...

cry (cryptochrome) is an important clock gene, and recent data indicate that it encodes a critical circadian photoreceptor in Drosophila. A mutant allele, cry(b), inhibits circadian photoresponses. Restricting CRY expression to specific fly tissues shows that CRY expression is needed in a cell-autonomous fashion for oscillators present in different locations. CRY overexpression in brain pacemak...

Methods Prospective, interventional study conduct in an academic surgical ICU. After local ethic committee agreement and consent (relatives and patients) Critically ill patients, intubated and mechanically ventilated, sedated, were included in the absence of ocular pathology, pace-maker, neuromuscular blockade agent, TBI or spinal cord injury. Before each nursing, the PDR was measured using a p...