MicroRNAs play important regulatory roles in eukaryotic lineages. In this paper, we employed deep sequencing technology to sequence and identify microRNAs in M. incognita genome, which is one of the important plant parasitic nematodes. We identified 102 M. incognita microRNA genes, which can be grouped into 71 nonredundant miRNAs based on mature sequences. Among the 71 miRANs, 27 are known miRN...

In India, small ruminants contribute in providing economic security to small, landless and marginal farmers. Parasitic diseases are important cause of production losses in small ruminants the world over. Of these, gastrointestinal (GI) nematodosis is a common parasitic infection of small ruminants in India including sub-Himalyan region of northern India (Yadav et al., 2009). It is caused by mix...

A PCR-DGGE primer pair, Tyl2F-Tyl4R, specific to plant parasitic and fungivorous nematodes was designed based on the 18S rRNA gene. The results of community analysis using the primers showed that they are specific to the order Tylenchida. This primer pair detected species belonging to Tylenchida with high sensitivity and high resolution. The number of detected species of plant parasitic and fun...

RNA interference (RNAi) is widely used in Caenorhabditis elegans to identify gene function and has been adapted as a high-throughput screening method to identify genes involved in essential processes. The technique has been applied to parasitic nematodes with variable success and we believe that inconsistent outcomes preclude its use as a robust screen with which to identify potential control t...

Plant-parasitic nematodes are major constraints to the productivity of tropical farming operations. Intensive land use and climatic conditions favorable to nematode development contribute to increased crop losses due to these pests. Many farmers in developing tropical countries have limited resources and management options. Cropping systems research is a relatively low-cost, low-input method of...

Root-knot nematodes represent one of the most important plant pathogens in Pomegranate cultivation regions. By studying structure plant-parasitic community inhabiting rhizosphere fields, it was found that

background: among the infectious organisms of parasitic origin, gastrointestinal nematodes are very important as they have been reported worldwide. the main aim of the present research study to highlight the annual epidemiological contributing factors associated with the prevalence of gastrointestinal nematodes and their control in sheep. methods: a total 1200 faecal samples (100 per month) wer...

Organotins (OTs) are globally ubiquitous contaminants. Contamination of OTs, particularly butyltins (BTs), has been suspected to cause immunosuppressive effects leading to subsequent infectious diseases or opportunistic infection by pathogens in marine mammals. In the present study, we collected individuals from five different populations of finless porpoises (Neophocaena phocaenoides) from coa...

BACKGROUND Dispersal is an important nematode behavior. Upon crowding or food depletion, the free living bacteriovorus nematode Caenorhabditis elegans produces stress resistant dispersal larvae, called dauer, which are analogous to second stage juveniles (J2) of plant parasitic Meloidogyne spp. and infective juveniles (IJ)s of entomopathogenic nematodes (EPN), e.g., Steinernema feltiae. Regulat...

microRNAs are small non-coding RNAs that are important regulators of gene expression in a range of animals, including nematodes. We have analysed a cluster of four miRNAs from the pathogenic nematode species Haemonchus contortus that are closely linked in the genome. We find that the cluster is conserved only in clade V parasitic nematodes and in some ascarids, but not in other clade III specie...