We introduce a methodology based on the Luria-Delbruck fluctuation model for estimating the cell kinetics of clonally expanding populations. In particular, this approach allows estimation of the net cell proliferation rate, the extinction coefficient and the initial (viable) population size. We present a systematic approach based on spatial partitioning, which captures the local fluctuations of...

A study was made to find out maximum partitioning of Bacillus licheniformis alkaline phosphatase in different ATPSs composed of different molecular weight of PEG X (X=2000, 4000, 6000) with salts (magnesium sulphate, sodium sulphate, sodium citrate) and polymers (dextran 40, dextran T500). Physicochemical factors such as effect of system pH, system temperature and production media were evaluate...

I consider the effect of a small concentration of a molecule, such as a short-chain alcohol, on the miscibility transition temperature of a giant plasma membrane vesicle. For concentrations sufficiently small such that the system can be treated as a weak solution, the change in transition temperature is known to depend upon the extent of the molecule’s partition into the coexisting liquid-disor...

rapeseed (brassica napus l.) is a crop relatively tolerant to salt. the effect of different ratios of nacl/kcl was studied in two cultivars (hyola308 and kristina) of rapeseed in 2002. canola seedlings were grown in hoagland’s solutions, which were salinized by different ratios of nacl/kcl. the treatments or kcl:nacl ratios were 0:0 (control);10:10; 20:0 (total added salt or tas=20 mol.m-3), 0:...

We consider preconditioned Krylov subspace methods for computing the stationary probability distribution vector of irreducible Markov chains. We propose preconditioners constructed as the product of two fairly simple preconditioners. Theoretical properties of the proposed product preconditioners are briefly discussed. We use graph partitioning tools to partition the coefficient matrix in order ...

I consider the effect of a small concentration of a molecule, such as a short-chain alcohol, on the miscibility transition temperature of a giant plasma membrane vesicle. For concentrations sufficiently small such that the system can be treated as a dilute solution, the change in transition temperature is known to depend upon the extent of the molecule's partition into the coexisting liquid-dis...

We consider preconditioned Krylov subspace methods for computing the stationary probability distribution vector of irreducible Markov chains. We propose preconditioners constructed as the product of two fairly simple preconditioners. Theoretical properties of the proposed product preconditioners are briefly discussed. We use graph partitioning tools to partition the coefficient matrix in order ...

Recent studies show the Earth’s core may contain more magnesium (Mg) than previously thought, with perhaps up to 6 wt% in the early core and ∼1wt% still existing now. The Mg partitioning between liquid and solid iron (Fe) under the relevant conditions is needed, therefore, in order to establish whether the presence of magnesium will have an effect on core properties, particularly those of the i...

background: phenylalanine dehydrogenase (phedh ec 1.4.1.20) is a nad+-dependent enzyme that performs the reversible oxidative deamination of l-phenylalanine to phenylpyruvate. it plays an important role in detection and screening of phenylketonuria (pku) diseases and production of chiral intermediates as well. the main goal of this study was to find a simple and rapid alternative method for pur...

This work aimed to optimize the parameters affecting partitioning of a bacteriocin-like inhibitory substances (BLIS) from Lactococcus lactis Gh1 in extractive fermentation using polyethylene glycol (PEG)/dextran aqueous two-phase system (ATPS). was developed for simultaneous cell cultivation and downstream processing BLIS. Results showed that molecular weight PEG, PEG concentration, dextran T50...