To understand the control of glial tumor cell proliferation, we have examined the effects of neurons on a number of human and rodent glioma lines. These included C6, G26-24, U-251, HTB-16, and A-172 cells of astroglial lineage and G26-20 of bipotential astrocytic and oligodendrocytic lineage. Rapid, specific binding of granule neurons to the human A-172, HTB-16, and U-251 and mouse G26-24 cell ...

INTRODUCTION Microfluidics systems usually consist of materials like PMMA--poly(methyl methacrylate) and PDMS--poly(dimethylsiloxane) and not polystyrene (PS), which is usually used for cell culture. Cellular and molecular responses in cells grown on PS are well characterized due to decades of accumulated research. In contrast, the experience base is limited for materials used in microfludics c...

Monoclonal antibodies specific for neurofilament (NF) subunits or for vimentin filament (VF) protein were used to study nerve growth factor (NGF)-induced intermediate filament expression and organization in a rat pheochromocytoma cell line (PC12 cells). NGF induced increased amounts of NF subunits and VF protein, and they were, in part, differentially localized within PC12 cells. The relative e...

Acid-sensing ion channels (ASICs) are expressed in various sensory and central neurons. The functional role of these channels remains elusive. Complex subunit combinations and lack of specific blockers for native receptors are likely to contribute to the difficulty of resolving the function of ASICs. Finding a neuronal cell line, which expresses a single population of ASICs, should prove to be ...

Aims To explicit cell apoptosis trend in PC12 oxygen-glucose-serum deprivation/restoration (OGSD/R) model and provide experimental bases for neural cell simulation in ischemia reperfusion injury in vitro. Methods OGSD/R model was constructed using the passage PC12 cells in vitro. The profile of cell apoptosis was estimated by DAPI staining, Annexin V-FITC and terminal deoxynucleotidyl transfe...

Neuronal responses to extracellular matrix (ECM) constituents are likely to play an important role in nervous system development and regeneration. We have studied the interactions of a neuron-like rat pheochromocytoma cell line, PC12, with ECM protein-coated substrates. Using a quantitative cell attachment assay, PC12 cells were shown to adhere readily to laminin (LN) or collagen IV (Col IV) bu...

BACKGROUND Parkinson's disease (PD) is the second most common neurodegenerative disease, affecting 2% of the population aged over 65 years old. Mitochondrial defects and oxidative stress actively participate in degeneration of dopaminergic (DA) neurons in PD. Paeonolum, a main component isolated from Moutan cortex, has potent antioxidant ability. Here, we have examined the effects of paeonolum ...

Background and objectives: Nigella sativa seeds possess anti-inflammatory, antioxidant, tranquillizer, chemoprotective and antineoplastic effects. They seeds are important nutritional flavoring agents and natural remedies for many aliments. We have investigated the protective effects of N. sativa seed fatty acids against doxorubicin. Methods: ...

The PC12 clone of pheochromocytoma cells undergoes neuronal differentiation in the presence of nerve growth factor (NGF). Concomitant with this is a significant induction in the incorporation of radiolabeled fucose or glucosamine into a 230,000-dalton cell surface glycoprotein named the NGF-Inducible Large External, or NILE, glycoprotein (GP) (McGuire, J. C., L. A. Greene, and A. V. Furano (197...

luteolin, as a natural polyphenolic compound, has neuroprotective effect and exerts its function by attenuation of apoptosis and oxidative stress factors. emerging evidences indicate that oxidative stress leads to neurodegeneration but is not the initial event and endoplasmic reticulum stress (er) is often considered to be the stimulus event which is caused by accumulating of misfolded proteins...