Escherichia coli isocitrate lyase (EC 4.1.3.1.) can be phosphorylated in vitro by an ATP-dependent reaction. The enzyme becomes phosphorylated by an endogenous kinase when partially purified sonic extracts are incubated with [gamma-32P]ATP. Treatment of isocitrate lyase with diethyl pyrocarbonate, a histidine-modifying reagent, blocked incorporation of [32P]phosphate from [gamma-32P]ATP. The is...

Pectate lyase genes have been documented as excellent candidates for improvement of fruit firmness. However, implementation of pectate lyase in regulating fruit postharvest deterioration has not been fully explored. In this report, 22 individual pectate lyase genes in tomato were identified, and one pectate lyase gene SlPL (Solyc03g111690) showed dominant expression during fruit maturation. RNA...

It is generally accepted that all of the allantoin-degrading enzymes (allantoinase, allantoicase, ureidoglycollate lyase and urease), used in purine degradation, were lost during mammalian evolution. However, surprisingly, ureidoglycollate lyase has been found in a mammalian tissue. Ureidoglycollate lyase was purified to homogeneity and characterized from rat-liver mitochondria. The apparent Km...

Alginate lyase mainly produces active alginate oligosaccharides (AOS) by degrading via β-elimination process. In this study, the Pseudoalteromonas sp. Alg6B lyase-encoding gene alg6B-7 from polysaccharide (PL)-7 family was successfully cloned, sequenced, expressed in Escherichia coli. Based on rational design and amino acid sequence alignment of various sources, four positive mutants were obtai...

To ascertain the role of pectin disassembly in fruit softening, chelated- (CSP) and sodium carbonate-soluble (SSP) pectins from plants with a pectate lyase, FaplC, or a polygalacturonase, FaPG1, downregulated by antisense transformation were characterized at the nanostructural level. Fruits from transgenic plants were firmer than the control, although FaPG1 suppression had a greater effect on f...

An improved enzymatic cotton scouring process has been developed on the basis of a fast enzyme reaction to efficiently remove the pectin and wax compounds from the organic cotton fabrics. An attempt has been made to study the pectinolytic activity of degrading rate of pectin on the organic cotton fabric using alkaline pectinase enzyme with various process parameters such as enzyme concentration...

In the preceding paper (1) a method was described for the purification of the extracellular pectin trans-eliminase of Aspergillus jonsecaeus. The enzyme was purified by a three-step procedure to the point at which other pectic enzymes and cellulase were no longer detectable. The effects of pH, of Na+, Mg++, and Ca++ ions, and of a few anions on the activity of the enzyme were also reported. In ...

The origin of root softening during cassava retting was investigated in a natural retting and in a sterile fermentation. Softening only occurred in the natural retting. Although high activities of endogenous pectin methyl esterase were found in cassava extracts from both fermentations, the depolymerizing enzymes polygalacturonase, active at low pH, and pectate lyase were only found in the non-s...

In 1960 Albersheim, Neukom, and Deuel (1) reported on an enzyme present in a commercial pectic enzyme preparation (Pectin01 RlO, Rohm and Haas Company, Philadelphia) that degraded the a, 1 + 4-glycokidic bonds in pectin by a trans elimination of the proton on the 5th carbon atom of an anhydromethyl galacturonate unit with the oxygen of the adjacent glycosidic bond. Cleavage of the bonds in pect...

gene that responds to DNA-damaging agents. J. Bacteriol. 172:3284-3289. McEvoy, J. L., K.K. Thum, and A.K. Chatterjee. 1987. Expression of the E. coli lexA+ gene in Erwinia carotovora subsp, carotovora and its effect on production of pectin lyase and carotovoricin. Fed. European Microbiol. Soc. Microbiol. Lett. 42:205–208. Mount, M.S., P.M. Berman, R.P. Mortlock, and J.P. Hubbard. 1979. Regulat...