Outer dense fibers (ODFs), as unique accessory structures in mammalian sperm, are considered to play a role in the protection of the sperm tail against shear forces. However, the role and relevant mechanisms of ODFs in modulating sperm motility and its pathological involvement in asthenozoospermia were unknown. Here, we found that the percentage of ODF defects was higher in asthenozoospermic sa...

Semen samples collected from 28 male partners of infertile couples were divided into three equal aliquots and prepared with three selected media, such as PureSperm (Nidacon, Gothenburg, Sweden), Sil-Select Plus (Fertipro, Beernem, Belgium) and SpermGrad (Vitrolife, Gothenburg, Sweden). The differences in mean percentages of semen parameters were assessed by repeated measures analysis. Correlati...

Fertilization and reproduction are central to the survival and propagation of a species. Couples who cannot reproduce naturally have to undergo in vitro clinical procedures. An integral part of these clinical procedures includes isolation of healthy sperm from raw semen. Existing sperm sorting methods are not efficient and isolate sperm having high DNA fragmentation and reactive oxygen species ...

background: the etiologic cause in near one third of male factor infertility is unknown. the percentage of men with idiopathic infertility who have been successfully treated by the empirical therapeutic modalities is not high. objective: the aim of this study was to assay the effect of l-carnitine on sperm parameters in patients who needs intracytoplasmic sperm injection (icsi) as a method for ...

The effects of acute restraint stress on sperm motility and reproductive endocrinology were investigated in adult rats. Sperm motility was determined by computer-assisted sperm analysis. Acute restraint stress reduced sperm motility starting after 30 min, and the sperm motility parameters, percentage of motile spermatozoa (%), straight-line velocity, curvilinear velocity, deviation of the sperm...

Herein we demonstrated the effect of oxygen and air atmosphere on the success of short-term storage of sperm obtained from rainbow trout (Oncorhynchus mykiss), sterlet (Acipenser ruthenus) and common carp (Cyprinus carpio) diluted in immobilizing media (100 mM NaCl, 40 mM KCl, 3 mM CaCl2, 1.5 mM MgCl2, 50mM Tris, pH 8.2 for salmonid fish, 17 mM NaCl, 1.7 mM KCl, 6 mM NaHCO3, 0.5 mM CaCl2, 0.5 m...

objective: the aim of this study was to evaluate the non-genomic action of thyroxin on sperm kinetic and its probable use to improve sperm recovery after applying an enrichment method like “swim-up” in comparison with the available one, pentoxifylline. materials and methods: this is an experimental study. a total of 50 patients were recruited, followed by infertility consultation. conventional ...

BACKGROUND We designed this study to detect the cryoinjury rate on human sperm after serial freezing and thawing, taking into consideration the effects of using cryovials and straws. MATERIALS AND METHODS In this experimental study, semen specimens obtained from 15 subjects were divided into normozoospermic and oligozoospermic groups. Each of the normozoospermic and oligozoo spermic semen spe...

background: sodium arsenite can impair male reproductive function by inducing oxidative stress. silymarin is known as a potent antioxidant.objective: this study was performed to investigate if silymarin can prevent the adverse effect of sodium arsenite on ram sperm viability, motility and mitochondrial membrane potential.materials and methods: epidydimal spermatozoa obtained from ram were divid...