High-performance liquid chromatography has replaced several spectroscopic methods and gas in the quantitative qualitative analysis of medications pre-sale procedure testing done past ten years. This is as part marketing drugs their control. During early stages its application, high-performance (HPLC) was expected to be a that would supplement chromatography; nevertheless, today, HPLC almost ent...

In recent years there has been considerable interest in the synthesis and separation of enantiomers of organic compounds especially because of their importance in the biochemistry and pharmaceutical industry. High-performance liquid Chromatography is a very useful method for the direct separation of enantiomers. However, about 30−40 years ago, commercially available chiral stationary phases wer...

Background and Objectives: Furfural (F) and hydroxymethyl furfural (HMF) are cyclic aldehydes, which are formed during the heat processing of foods. These chemical contaminants have received much attention due to their suspected health hazards and heat damage indicators. The aim of the present study was extraction and determination of F and HMF in baby-foods using dispersive liquid-liquid micro...

Background and Objectives: A method was developed and validated for determining 15 polycyclic aromatic hydrocarbons (PAHs) in refined olive and refined pomace olive oils using high performance liquid chromatography coupled with a fluorescence detector. Materials and Methods: The sample preparation involved liquid–liquid extraction with organic solvent and low temperature clean-up. The low temp...

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High-performance liquid chromatography (HPLC) is a refinement of traditional column chromatographic techniques. The speed of analysis and the resolution are increased with new column-packing materials and eluant pumped through the column at high pressure. The potential for achieving measurements of hormones in small volumes of plasma or urine is limited, both in normal and pathological situatio...

In the present study, various mixtures of aqueous ethanol and acetone solutions were utilized to extract anthocyanins from eggplant peel by a discontinuous process to obtain a natural red-purple colorant. First, the most appropriate combination for extraction solvent was selected as ethanol 70 wt in water acidified with acetic acid 3 wt, which was determined by analysis of extraction yields and...

Michael E. Swartz, Ph.D. Principal Scientist, Waters Corporation, Milford, Massachusetts, e-mail [email protected]. igh performance liquid chromatography (HPLC) is a proven technique that has been used in laboratories worldwide over the past 30-plus years. One of the primary drivers for the growth of this technique has been the evolution of packing materials used to effect the separatio...

A reversed-phase system is described for the simultaneous isocratic separation of coproporphyrin I, II, III and IV isomers. The retention behaviour of coproporphyrin I and III is studied in detail. The method is suitable for both analytical and semi-preparative separation.