The function of middle hepatitis B surface protein C‑terminally truncated at amino acid position 167 (MHBst167) is not currently clear. This study aimed to screen and identify the proteins that interact with MHBst167 in hepatocytes using a yeast two‑hybrid system, and to explore the effects of MHBst167 in the development of hepatocellular carcinoma and precancerous diseases of the liver. The MH...

human growth hormone (hgh) genomic sequence containing 5 exons and 4 introns was cloned in pcdna-3 and the constructed plasmid was subsequently used for transfection ofnlli-3t3 cell line using lipofection technique. expression of hgh in stably transfected cells was assayed using elisa. total rna was extracted from transfected cells and hgh cdna was amplified by rt-pcr using specific primers. th...

EcoRI polymorphism within the GLI gene (chromosome 12q13.3-14.1) Source/Description: pKK36Pl contains a 1.55 kb PstI genomic fragment of GLJ cloned into the PstI site of pGEM-3 (1). Polymorphism: EcoRI identifies a two allele polymorphism (Al: 18 kb; A2: 5 kb). Frequency: Estimated from 91 individuals. Al 18 kb (0.72) A2 5kb (0 28) Chromosomal Localisation: Assigned to chromosome 12ql3.3-14.1 u...

A spontaneous fluoroquinolone-resistant mutant (STM1) was isolated from its parent Salmonella enterica serovar Typhi (S. Typhi) clinical isolate. Unlike its parent isolate, this mutant has selective resistance to fluoroquinolones without any change in its sensitivity to various other antibiotics. DNA gyrase assays revealed that the fluoroquinolone resistance phenotype of the STM1 mutant did not...

BACKGROUND Few studies have attempted to understand the complexity of microbial populations in Pakistan where infectious diseases are prevalent. This study was undertaken to assess bacterial biodiversity in Nehr-e-Khayyam a heavily polluted stream connected to the Arabian Gulf, which runs through a densely populated urban area in Karachi, Pakistan. METHODS Employing a universal pair of oligon...

Sequencing of Buchnera amino acid genes Amplification primers (table S2) were designed using the sequenced genome of Buchnera APS (NC_002528.1) and synthesized by Eurofins-MWG Operon. PCR conditions were an initial denaturing step at 94° C for 2 min, followed by 30 cycles of 94° C for 30 s, 54-60o C depending on primer Tm for 30 s, 72° C for 2-4 min depending on amplicon length, and a final ext...

1,4-Dihydroxy-2-naphthoic acid (DHNA), a precursor of menaquinone (vitamin K2), has an effect on growth stimulation of bifidobacteria and prevention of osteoporosis, making it a promising functional food material. Therefore, we tried to clone the menB gene encoding DHNA synthase from Leuconostoc mesenteroides CJNU 0147. Based on the genome sequence of Leu. mesenteroides ATCC 8293 (GenBank acces...

Ornithine decarboxylase (OIK ) belongs to a multigene family and some of these may very well be nonfunctional (pseudogenes). We isolated an ODC gene from a human chromosome 2-specific library and transfected the gene into ODC-deficient Chinese hamster ovary cells to directly demonstrate that this ODC gene is functional and OIK is essential for cell proliferation. After screening 2.5 x 10* plaqu...

The variability of the 5' untranslated genomic region (5'UTR) of bovine viral diarrhoea virus (BVDV) RNA obtained from a single individual was analysed. Lung, kidney and spleen tissues from a naturally infected foetus were used as the source of viral RNA. A fragment of 288 bases of the internal ribosome entry site from the BVDV 5'UTR was amplified by RT-PCR using a proofreading DNA polymerase. ...

1The authors thank A. L. Archibald, M. F. Rothschild, and the EC PiGMaP group for distribution of DNA from the PiGMaP reference families, and D. Nicholson, C. Haley, and G. J. Lee for provision of database services and linkage analyses. Published as paper no. 11935, Journal Series, Agric. Res. Div., Univ. of Nebraska, Lincoln 68583-0908. 2To whom correspondence should be addressed: phone: (402)...