human serum albumin (hsa) is an important protein that carries variety of substances like some hormones and drugs in blood. pharmacological studies of the interaction of many drugs and hsa are reported during several decades, specially recently years. interaction of cortisol and fluoxetine hydrochloride (flx) (as a common anti-stress drug) with hsa (as their carrier in blood) has been studied s...

I t was noted by Mellanby and Wooley (1) that trypsin solutions in dilute acid could be heated nearly to boiling with very little loss in activity, and this observation was confirmed by Eddie (2). At temperatures below 40°C., on the other hand, the enzyme is more stable near pH 4 or 5 than it is in acid solution. This latter result has also been obtained by the writer (3) and by Pace (4). In th...

Enzymes that are used as animal feed supplements should be able to withstand temperatures of 60 to 90 degrees C, which may be reached during the feed pelleting process. The thermostability properties of three histidine acid phosphatases, Aspergillus fumigatus phytase, Aspergillus niger phytase, and A. niger optimum pH 2.5 acid phosphatase, were investigated by measuring circular dichroism, fluo...

Catabolic dehydroquinase which functions in the inducible quinic acid catabolic pathway in Neurospora crassa has been purified 8000-fold. The enzyme was purified by two methods. One used heat denaturation of contaminating proteins; the other used antibody affinity chromatography. The preparations obtained by these two methods were identical by all criteria. The purified enzyme is extremely resi...

The pH-dependent transitions and the thermal unfolding of defatted human serum albumin in 0.2 M KC1 as followed by polarimetry at 233 nm are found to be independent processes. The apparent net loss of structure (01 helix) in the unfolding between pH ‘7 and 9 is 2.5%, in the N-F transformation it is 8% and in the acid expansion 1%. The two last mentioned transitions seem to be independent of eac...

The pH-dependent transitions and the thermal unfolding of defatted human serum albumin in 0.2 M KC1 as followed by polarimetry at 233 nm are found to be independent processes. The apparent net loss of structure (01 helix) in the unfolding between pH ‘7 and 9 is 2.5%, in the N-F transformation it is 8% and in the acid expansion 1%. The two last mentioned transitions seem to be independent of eac...

NaClO(4) was employed in a technique for the rapid extraction of reovirus ribonucleic acid (RNA). The extracted RNA, which was purified in a Cs(2)SO(4) equilibrium density gradient, had a buoyant density of 1.61 g/cm(3) and a sedimentation coefficient of 15S in a 7 to 20% sucrose gradient. It was 90% resistant to ribonuclease in a solution of high ionic strength (0.1 m NaCl). The sensitivity of...

in the present study, the interaction between nano-tio2 and lysozyme was investigated by the method of uv-vis detection and fluorescence spectroscopic techniques. the thermal denaturation of lysozyme has been investigated in the presence and absence of nano-tio2 over the temperature range (293-373) k in different buffer and ph, using temperature scanning spectroscopy. the presence of nano-tio2 ...

The predictive value of sperm chromatin integrity for pregnancy outcome following in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) was studied in 24 men attending a university-based assisted reproductive techniques laboratory using the flow cytometric sperm chromatin structure assay (SCSA). The SCSA is a measure of the susceptibility of sperm DNA to low pH-induced denatu...

The interaction between nano-SiO2 and lysozyme was investigated by the method of UV-Visible  detection and fluorescence spectroscopic techniques. The thermal denaturation of lysozyme has been investigated in the presence and absence of nano-SiO2 over the temperature range (293-373) K in different buffers and pH values, using temperature scanning spectroscopy. The presence of nano-SiO2 caused th...