Transglutaminase (TGase) is widely used in the food industry. In this study, TGase was purified by affinity precipitation using l-thyroxin, coupled to a thermo-responsive polymer (PNBN), as an affinity ligand. The lower critical solution temperature and recovery of the affinity polymer were 31.0 °C and 99.6 %, respectively. The optimal adsorption condition was 0.02 mol/L phosphate buffer (pH 5....

Polymeric networks that have inherent capabilities to recognize different molecules and chemical changes in their environment are the next generation of materials that will aid in the diagnosis and treatment of diseases. We have prepared new networks based on star polymers that were designed to be responsive and recognitive. Using molecular imprinting with D-glucose and crosslinking with poly(e...

The design and synthesis of a novel acid-degradable polyethylene glycol-based N-hydroxysuccinimide (NHS) ester-activated crosslinker is reported. reactive towards nucleophiles features central ketal functional group that stable at pH > 7.5 rapidly hydrolyses 6.0. used to (i) fabricate polysaccharide hydrogels exhibit controlled degradation upon exposure an acidic environment or via endogenous e...

Alkynyl-terminated double hydrophilic ABC triblock copolymer, poly(oligo(ethylene glycol) monomethyl ether methacrylate)-b-poly(2-(dimethylamino) ethyl methacrylate)-b-poly(2(diethylamino) ethyl methacrylate) (alkynyl-POEGMA-b-PDMA-b-PDEA), was synthesized via atom transfer radical polymerization (ATRP) by sequential monomer addition using propargyl 2-bromoisobutyrate (PgBiB) as the initiator. ...

pH sensor is an important and practical device with a wide application in environmental protection field and biomedical industries. An efficient way to enhance the practicability of intelligent polymer composed pH sensor is to subtilize the three-dimensional microstructure of the materials, adding measurable features to visualize the output signal. In this work, C. rubi wing scales were combine...

(1) Background: Cytoplasmic delivery of antigens is crucial for the induction of cellular immunity, which is an important immune response for the treatment of cancer and infectious diseases. To date, fusogenic protein-incorporated liposomes and pH-responsive polymer-modified liposomes have been used to achieve cytoplasmic delivery of antigen via membrane rupture or fusion with endosomes. Howeve...

Progress in the development of generic molecular devices based on responsive polymers is discussed. Characterisation of specially synthesised polyelectrolyte gels, "grafted from" brushes and triblock copolymers is reported. A Landolt pH-oscillator, based on bromate/ sulfite/ferrocyanide, with a room temperature period of 20 min and a range of 3.1 < pH < 7.0, has been used to drive periodic osci...

We have engineered a new class of pH-responsive polymer films on gold surfaces by first developing a controlled, surface-catalyzed polymerization to prepare a copolymer film consistent with poly(methylene-co-ethyl acetate) and subsequently hydrolyzing the ester side chains to varying extents to yield carboxylic acids (denoted as PM-CO2H). When pH is increased, the acid groups become deprotonate...

Stimuli-responsive polymer hydrogels which are capable of responding to changes in their environment such as pH (1), osmotic strength (2), or temperature (3) have received growing attention in recent years for applications in a wide variety of areas including controlled drug delivery (4). Also, due to the size limitations imposed by the body’s natural defense mechanisms, such as the reticuloend...