A cDNA clone encoding a manganese-dependent peroxidase from the filamentous fungus Phanerochaete chrysosporium was isolated and characterized. The clone, AMP-1, was isolated by screening a X g t l l expression library with polyclonal antibodies raised against a purified manganese-dependent peroxidase (isozyme H4, PI 4.5). The XMP-1 cDNA sequence predicts a mature protein containing 358 amino a...

The ability of Phanerochaete laevis HHB-1625 to transform polycyclic aromatic hydrocarbons (PAHs) in liquid culture was studied in relation to its complement of extracellular ligninolytic enzymes. In nitrogen-limited liquid medium, P. laevis produced high levels of manganese peroxidase (MnP). MnP activity was strongly regulated by the amount of Mn2+ in the culture medium, as has been previously...

Two families of peroxidases-lignin peroxidase (LiP) and manganese-dependent lignin peroxidase (MnP)-are formed by the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium and other white rot fungi. Isoenzymes of these enzyme families carry out reactions important to the biodegradation of lignin. This research investigated the regulation of LiP and MnP production by Mn(II). In li...

The major cellobiose dehydrogenase (oxidase) (CBDH) secreted by the soft-rot thermophilic fungus Humicola insolens during growth on cellulose has been isolated and purified. It was shown to be a haemoflavoprotein with a molecular weight of 92 kDa and a pI of 4.0, capable of oxidizing the anomeric carbon of cellobiose, soluble cellooligosaccharides, lactose, xylobiose and maltose. Possible elect...

the sorption behavior of loofa-immobilized phanerochaete chrysosporium mycelia in two forms, live (l) and heat-inactivated (hia), was studied for the removal of hg2+ ions from aqueous solution. using the langmuir isotherm, the two key parameters for the sorption performance, qm and the coefficient b, were obtained; the qm values for hg2+ ions were 72.46 mg/g and 92.59 mg/g and the b coefficient...

the biosorption of heavy metals can be an effective process for the removal of such metal ions from aqueous solutions. in this study, the adsorption properties of nonliving biomass of phanerochaete chrysosporium for pb (ii) and zn (ii) were investigated by the use of batch adsorption techniques. the effects of initial metal ion concentration, initial ph, biosorbent concentration, stirring speed...