Microbial bioremediation of petroleum wastewater by phenol-degrading-bacteria holds promise in circumventing the issue of petroleum-spill related pollution. Herein, biodegradation of petroleum wastewater samples collected from oil refinery site was carried out in a fluidized bed bioreactor by Ca-alginate immobilized biomass of phenol-degrading strains of Bacillus cereus (AKG1 MTCC9817 and AKG2 ...

In this work the aerobic degradation of phenol (PH), catechol (CA), resorcinol (RE), hydroquinone (HY) and of the binary mixtures PH+CA, PH+RE, PH+HY by phenol-acclimated activated sludge was studied. Single substrate experiments show a Haldane-type dependence of the respiration rate on PH, RE and HY, while CA corresponded to the Monod model. Binary substrate experiments demonstrated that the p...

A bacterium, Pseudomonas aeruginosa (ATTC27853), was investigated for its ability to grow and to degrade phenol as sole carbon source, in aerobic batch culture. The parameters which affect the substrate biodegradation such as the adaptation of bacteria to phenol, the temperature, and the nature of the bacteria were investigated. The results show that for a range of temperature of 30 to 40°C, th...

Phenols have been recognized as mutagenic and carcinogenic. Industrial effluents possessing phenols causes’ environmental pollution. Present study was planned to investigate the % degradation of phenol using alkaliphilic Bacillus badius D1 with various concentration ranging from 0.42 to 1.68 g/L in alkaline broth of pH-9, in shaking incubator at110 rpm, 37 0 C. The % degradation at lowest conce...

The environment, as a consequence of industrial and agricultural revolutions, tends to harden with potentially carcinogenic and mutagenic halogen-substituted aromatic compounds. Phenol and its higher molecular homologues are dangerous environmental pollutants. Due to their toxic character, these molecules tend to accumulate in water and soil after being discharged without an adequate treatment....

In this study a mathematical model consisting of mass balance equation and accounting for reaction, mass transfer is developed to describe unsteady state phenol degradation in biofilter. For simulation purpose software BIOPHEN was developed in the language ForTran 77 and subsequently solved using Backward Implicit Scheme. The removal efficiency of phenol at inlet concentration is 99.47%. First ...

Taxonomic identification of three newly isolated Antarctic fungal strains by their 18S rDNA sequences revealed their affiliation with Aspergillus fumigatus. Phenol (0.5 g/l) as the sole carbon source was completely degraded by all strains within less than two weeks. Intracellular activities of three key enzymes involved in the phenol catabolism were determined. Activities of phenol hydroxylase ...

Catalase is one of the most potent biocatalyst which can oxidize potentially harmful toxins. Catalase is an abundant natural enzyme which is used in food industry, analytical applications (bio sensor) and textile industry to remove pollutants because of its high activity. Since catalase has many industrial applications, immobilization of catalase in solid support makes it heterogeneous catalyst...

(Received: June 5, 2011; Accepted: July 29, 2011) Abstract: Effect of increase in phenol concentration on performance of anaerobic baffled reactor in terms of chemical oxygen demand (COD), phenol and COD removal efficiency and biogas productions was investigated. Furthermore, stability of the reactor with respect to the chemical shock load was monitored. The anaerobic baffled reactor was contin...

Two fosmid libraries, totaling 13,200 clones, were obtained from bioreactor sludge of petroleum refinery wastewater treatment system. The library screening based on PCR and biological activity assays revealed more than 400 positive clones for phenol degradation. From these, 100 clones were randomly selected for pyrosequencing in order to evaluate the genetic potential of the microorganisms pres...