نتایج جستجو برای: phosphatidate phosphohydrolase
تعداد نتایج: 1031 فیلتر نتایج به سال:
The mechanism by which bi-and trivalent cations affect human liver phosphatidatephosphohydrolase (PAP) activity was investigated. Bivalent cations up to 1 mM increased PAP activity whereas at higher concentrations the activity of the enzyme decreased. The stimulatory concentration for trivalent cations such as Al3+ and Cr3+, however, was much lower being 2 m M and 1 m M, respectively. All catio...
Studies on the effects of garlic (Allium sativum) on hyperlipidemia have demonstrated somewhat controversial results and there have been few studies on its enzymatic mechanism. The purpose of this study was to assess the effect of garlic on the liver phosphatidate phosphohydrolase (PAP) activity, plasma lipid levels, malondialdehyde (MDA) and plasma antioxidant in rats fed either by normal or h...
Phosphatidate phosphohydrolase (PAP) from cytosolic fraction of rat liver was purified to homogeneity having specific activity of 5.14 U/mg protein. An activity staining procedure was developed to determine molecular weight of the enzyme on polyacrylamide gel electrophoresis using Ferguson plot. Molecular Weight (M.W.) of the active PAP was 298 KDa. SDS-PAGE analysis showed a M.W. of 47 KDa for...
The effects of therapeutic doses of dillsun, garsin, antum and statins on rat liver cytosolic phosphatidate phosphohydrolase (PAP) activity, a key enzyme in triacylglycerol synthesis, and on serum and liver lipids were examined. Lovastatin and simvastatin both stimulated the enzyme activity by 29% and 43%, respectively. The stimulatory effects were dose-dependent and accompanied by the decline ...
Momordica charantia is often called bitter melon, bitter gourd or bitter squash because its fruit has a bitter taste. The fruit has been widely used as vegetable and herbal medicine. Alpha-eleostearic acid is the major fatty acid in the seeds, but little is known about its biosynthesis. As an initial step towards understanding the biochemical mechanism of fatty acid accumulation in bitter melon...
Rat hepatocytes were treated with Staphylococcus aureus alpha-toxin to permeabilize their plasma membrane for low-molecular-mass compounds. During incubation with 1 mM labelled fatty acid, phosphatidate and, less clearly, lysophosphatidate rapidly reached a steady state, whereas labelled diacylglycerol accumulated to some extent, at least in the absence of exogenous CDP-choline. Esterification ...
Phospha t i d a t e p h o s p h o h y d r o l a s r (PAH) c a t a l y s e s t h e c o n v e r s i o n o f phospha t i d a t e t o d i a c y l g l y c r r o l and is a n i m p o r i a n t r e g u l a t o r y s t e p i n t r i a c y l g l y r e r o l synthesis. Long t e r m c o n t r o l o f enzyme a c t i v i t y is m e d i a t r d by hormones r i t h e r v i a i n c r r a s e d r n z y m c s y ...
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