Using in vitro techniques and labeled linoleic acid and glucose, alterations in phospholipid synthesis in the aorta were correlated with electron microscopic studies at various intervals of time after feeding rabbits cholesterol. After 4 to 8 weeks of feeding, more phospholipid precursors were incorporated into die phospholipids of atherosclerotic blood vessels than of normal vessels. Concomita...

The solid polysulfone made of SO2 and methylidenecyclopentane catalyzes the cleavage of methyl-substituted allyl ethers and liberates the corresponding alcohols.

A newly synthesized phospholipid, l,2-0-Dipentadecylmethylidene-glycerol-3-phosphoryl-(N-ethylamino) -ethanolamine, having positive charge was found to exercise fusion activity on plant proto­ plasts. This phospholipid is not found in cells and has an unusal chemical structure with a dioxo­ lane ring. Some properties of this compound are discussed in relation to phospholipid-induced cell fusion.

The distribution of phospholipid types between the two leaflets of a membrane bilayer is a controlled feature of membrane structure. One of the two membrane catalytic activities governing this distribution randomizes the composition of the two leaflets-the phospholipid scramblases. Two proteins (Xkr8 and TMEM16F) required for the activation of these activities have been identified. One of these...

Serum phospholipid levels were determined in 1,067 healthy persons aged 2 to 77 years. Phospholipid levels increased with age but in a different way for each of the sexes. The age-sex changes of serum phospholipids were similar to those observed for serum cholesterol. Correlation coefficients in members of 156 families indicated that the genetic factor is of importance in determining the serum ...

BACKGROUND Baohuoside I is a potential anticancer drug for a variety of malignancies and has been approved for in vitro use. However, baohuoside I has very poor oral absorption. METHODS In the present study, we prepared baohuoside I-phospholipid complexes of different diameters and determined their physicochemical properties using transmission electron microscopy, ultraviolet spectroscopy, an...

The in vivo metabolic pathways of phospholipid biosynthesis in Rhodopseudomonas sphaeroides have been investigated. Rapid pulse-chase-labeling studies indicated that phosphatidylethanolamine and phosphatidylglycerol were synthesized as in other eubacteria. The labeling pattern observed for N-acylphosphatidylserine (NAPS) was inconsistent with the synthesis of this phospholipid occurring by dire...

Cell-free protein synthesis is becoming a serious alternative to cell-based protein expression. Cell-free systems can deliver large amounts of cytoplasmic recombinant proteins after a few hours of incubation. Recent studies have shown that membrane proteins can be also expressed in cell-free reactions and directly inserted into phospholipid membranes. In this work, we present a quantitative met...

The ability of canine pancreatic signal peptidase to remove the signal peptide portion of presecretory proteins in a translocation-independent assay is shown to require phospholipid. Sodium deoxycholate extracts of canine pancreatic rough microsomes containing both signal peptidase and phospholipid were delipidated by gel filtration chromatography on Sepharose CL-6B equilibrated with 0.2% deoxy...

Prolongation of all phospholipid-dependent coagulation tests was found in a patient with macroglobulinemia, despite absence of bleeding manifestations. The purified monoclonal IgM lambda protein and its Fabmu tryptic fragment induced similar changes in normal plasma. Patient IgM and Fabmu completely inhibited Ca++-dependent binding of radiolabeled prothrombin and Factor X to mixed phospholipid ...