The purpose of this study was to examine host distribution patterns among fecal bacteria in the order Bacteroidales, with the goal of using endemic sequences as markers for fecal source identification in aquatic environments. We analyzed Bacteroidales 16S rRNA gene sequences from the feces of eight hosts: human, bovine, pig, horse, dog, cat, gull, and elk. Recovered sequences did not match data...

Materials and methods: On 15 British farms, invertebrate communities were trapcollected (14 per year), counted, and sorted into species groups. Lawsonia serological tests were conducted and total DNA was extracted from pig feces; DNA was also extracted from adult flies, pupae, and larvae samples and viscera of Blatta species. Each DNA sample was tested for L intracellularis by polymerase chain ...

The metabolism and disposition of /V-|4-(5-nitro-2-furyl)-2-thiazolyl|formamide (FANFT) and 2-amino-4-(5-nitro-2-furyl)thiazole (ANFT) were studied in rat and guinea pig. Rat is susceptible whereas guinea pig is resistant to FANFT-induced bladder cancer. Rats and guinea pigs were P.O.administered either 2-|14C|ANFT or 2-|'4qFANFT (100 mg/kg), and 18-h urine and feces were collected. Tissue dist...

Easy access to next generation sequencing has enabled the rapid analysis of complex microbial populations. To take full advantage of these technologies, animal models enabling the manipulation of human microbiomes and the study of the impact of such perturbations on the host are needed. To this aim we are developing experimentally tractable and clinically relevant pig models of the human adult ...

The metabolism and disposition of N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT) and 2-amino-4-(5-nitro-2-furyl)thiazole (ANFT) were studied in rat and guinea pig. Rat is susceptible whereas guinea pig is resistant to FANFT-induced bladder cancer. Rats and guinea pigs were p.o. administered either 2-[14C]ANFT or 2-[14C]FANFT (100 mg/kg), and 18-h urine and feces were collected. Tissue dis...

materials and methods stool specimens from a random population sample were examined with light microscope, using direct fecal smear, formalin-ether concentration, and nutrient agar plate culture. results the prevalence of s. stercoralis in this study was 0.07%. the statistical random sample in this study was 2839 people whose stool specimens were collected and examined using nutrient agar plate...

Ammonia (NH) volatilization from intensive livestock production is a threat to natural ecosystems. This study investigated pig diet manipulation by 1% (w/w) benzoic acid (BA) amendment and lowering of dietary electrolyte balance through substituting 1.4% (w/w) CaCO with 2.0% (w/w) CaCl. Urine and feces were collected separately from 24 pigs fed one of four diets (Control, +BA, +CaCl, +BA+CaCl) ...

Thirty-five isolates from pig feces were identified as Lactobacillus reuteri (12 strains), Lactobacillus mucosae (7), Lactobacillus plantarum (6), Lactobacillus kitasatonis (3), Lactobacillus rossiae (2), Lactobacillus ultunensis (2), Lactobacillus crispatus (2), and Lactobacillus intestinalis (1) by partial sequence analysis of the 16S rRNA. All isolates were detected at 8-9 log CFU g(-1). Pre...

This study investigated the amount of energy available to growing pigs from fermentation of dietary fiber in the hindgut. Eighteen growing barrows, fitted with a simple T-shaped cannula at the terminal ileum, were allocated to 3 experimental diets in a completely randomized design. The 3 diets were a standard-fiber diet (SFD), which contained 75.1 g NDF/kg diet; a medium-fiber diet (MFD) of 105...

Midwest U.S. herds (n=63) were studied to identify risk factors for harboring Salmonella enterica among slaughter-weight pigs. Samples collected on farms (feces) and at slaughter (distal colonic content, cecal content and ileocolic lymph nodes) were cultured using conventional means. Approximately 15 pigs were studied per herd, for a total of 3754 samples. The proportion of pigs positive in one...