A sequence S is potentially Km−Pk graphical if it has a realization containing a Km −Pk as a subgraph. Let σ(Km −Pk, n) denote the smallest degree sum such that every n-term graphical sequence S with σ(S) ≥ σ(Km−Pk, n) is potentially Km−Pk graphical. In this paper, we prove that σ(Km−Pk, n) ≥ (2m−6)n−(m−3)(m−2)+2, for n ≥ m ≥ k + 1 ≥ 4. We conjectured that equality holds for n ≥ m ≥ k + 1 ≥ 4. ...

The effects of hemin on the conversion of pyruvate kinase (PK) isozymes from the M2-type to the L-type in K562 cells were investigated. Immunofluorescence, ion exchange chromatography, and electrophoretic studies showed that the untreated K562 cells contained only the M2-type PK, while eight to 20 days after induction with hemin, concomitant with hemoglobin F synthesis, L-type PK levels increas...

A spectrophotometric method is used to determine the acidity constants of some derivatives of 9,10-anthraquinone in binary methanol-water mixtures at 25 °C. There is a linear inverse relationship between pKa of all acids and the mole fraction of methanol in the mixed solvent. The influence of substituents in the molecular structure on the ionization constants is discussed.  

Abstraet-l . The effects of the amino acid, aspartate, as an allosteric modifier of pyruvate kinase (PK) were surveyed in tissue extracts from 18 species of animals. 2. Aspartate was a strong activator of PK in selected tissues of gastropod and bivalves molluscs, particularly amongst anoxia-tolerant species; with one exception, a polychaete worm, aspartate had no effect on PK from non-molluscan...

The coding sequence of the cytosolic isozyme of potato tuber pyruvate kinase (PK) was attached to the transit peptide of the small subunit of pea ribulose-1,5-bisphosphate carboxylase oxygenase and placed under the control of the cauliflower mosaic virus 35S promoter. This construct was transformed into Nicotiana tabacum. Unexpectedly, two primary transformants were recovered in which PK activi...

BACKGROUND The synthetic potential chemotherapeutic agent 3-Chloro-4-[(4-methoxyphenyl) amino]furo[2,3-b]quinoline (PK-L4) is an analog of amsacrine. The half-life of PK-L4 is longer than that of amsacrine; however, PK-L4 is difficult to dissolve in aqueous media, which is problematic for administration by intravenous injection. AIMS To utilize solid lipid nanoparticles (SLNs) modified with p...

OBJECTIVE Develop a detailed representation of pharmacokinetics (PK), derived from the information in Summaries of Product Characteristics (SPCs), for use in computerized systems to help practitioners in pharmaco-therapeutic reasoning. METHODS Available knowledge about PK was studied to identify main PK concepts and organize them in a preliminary generic model. The information from 1950 PK SP...

Mammalian pyruvate kinase (PK) is a four-domain enzyme that is active as a homo-tetramer. Tissue-specific isozymes of PK exhibit distinct levels of allosteric regulation. PK expressed in muscle tissue (M1-PK) shows hyperbolic steady-state kinetics, whereas PK expressed in kidney tissue (M2-PK) displays sigmoidal kinetics. Rabbit M1 and M2-PK are isozymes whose sequences differ in only 22 out of...

To clarify the molecular abnormality of pyruvate kinase (PK) deficiency identified in the mutant mice of CBA-Pk-1slc/Pk-1slc, we cloned murine red blood cell-type PK (R-PK) cDNA of those animals. The cDNA sequence spans 1827 bp, including an open reading frame that can encode 574 amino acids. Homology in the coding sequences between murine and human R-PK was 86.1% at nucleotide and 91.5% at ami...