Gibberellic acid (GA3) is an important phytohormone, a member of gibberellins family, which acts as a promoter and regulator of plant growth. This study aimed to evaluate GA3 production by Fusarium moniliforme LPB03 and Gibberella fujikuroi LPB06 using different techniques of fermentation, solid state fermentation (SSF), submerged fermentation (SmF), and semisolid state fermentation (SSSF), and...

Anaerobic digestion can be used to degrade food waste and recover energy. Methane is a biogas that can be efficiently converted in electricity. Organic loading rate, temperature, time, pH, carbon to nitrogen ratio are important factors to be operated in the bioreactors and still are challenges in this process to increase biogas production. It has been reviewed the single phase and two-stage bio...

The scale-up of stirred tank bioreactors from 0.02 m3 to a 03 m-’ commercial plant is discussed for hybridoma suspension cultures. Schemes for dissolved oxygen control with sparged air in serum containing media are described, as well as mechanical breakage offoam in small and large bioreactors. Porous metal spargers (180-200 x lo6 m) were found to produce foams which were hard to control. Aerat...

Coffee somatic embryogenesis in liquid medium is a powerful alternative to other vegetative propagation techniques for mass propagation of selected Coffea canephora (Robusta) clones and F1 Coffea arabica hybrids. This review presents the different types of bioreactors used for coffee somatic embryogenesis by Nestlé R&D Centre-Tours and by other scientific teams. Mechanically agitated bioreactor...

Recent papers have described new bioreactor designs. Most innovations addressed either oxygen transfer, shear induced by stirring, control of water activity in organic phase systems or waste biotreatment. Innovations made during the past year were reported in mainly three areas: bioreactor designs for increases in oxygen transfer and decreases in shear stress; bioreactors for two-phase reaction...

Adoptive cell transfer immunotherapy against melanoma is highly effective. However, this therapy has seen limited dissemination, mainly due to the complexity and costs of cell expansion protocols. Two bioreactors have recently been described that simplify and streamline the production of individualized cell therapies. Such bioreactors might increase the number of patients that get access to thi...

The low-shear, microgravity-simulating cell culture environment in Rotating Wall Vessel (RWV) Bioreactors RWV Bioreactors is well suited for generating differentiated 3-D tissue constructs (organoids). Biochemical, immunological and molecular biological techniques, including cDNA arrays, were used to analyze signal transduction, catecholamine contents, and gene expression in 3-D tissue-like con...

The production of recombinant biopharmaceuticals (pharmaceutical proteins) is a strongly growing area in the pharmaceutical industry. While most products to date are produced in mammalian cell cultures, namely Chinese hamster ovary cells, plant-based production systems gained increasing acceptance over the last years. Different plant systems have been established which are suitable for standard...