Shoot tips from actively growing, greenhouse maintained plants of Kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on Murashige and Skoog (MS) basal medium supplemented with NAA and BA, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. Results showed that the maximum plantlets height (7.012 cm), node number (4.516), root number (8.860) and roo...

Development of new techniques for in vitro culture is necessary to optimize and reduce the production costs. The aim of this work was to introduce the bioreactor BIB (Bioreactor of Immersion by Bubbles) and compare it with conventional culture and bioreactor RITA (Recipient with Automatized Temporary Immersion) for In vitro growth of M. alternifolia plantlets. Plantlets with 1.0 cm height from ...

The banana aphid, Pentalonia nigronervosa Coquerel, is present worldwide where banana (Musa spp.) is grown. It is the vector of Banana bunchy top virus (Nanoviridae, Babuvirus), the etiological agent of banana bunchy top disease, currently the most important constraint for banana producers in Hawaii. P. nigronervosa is not well studied, and effects of temperature on its growth and reproduction ...

This work describes an improved and efficient method for optimum maturation and normal embryo growth to produce healthy plantlets through somatic embryogenesis using suspension cultures for date palm cv. sakkoty. cell suspensions were supplemented with low amounts of dichlorophenoxyacetic acid (0.5mg/1) and different concentrations of abscise acid (AbA) (0.1, 0.3 and 0.5 mg/l) and polyethylene ...

The essential oils (EOs) and static headspaces (HSs) of in vitro plantlets and callus of Mentha x piperita were characterized by GC-MS analysis. Leaves were used as explants to induce in vitro plant material. The EO yields of the in vitro biomass were much lower (0.1% v/w) than those of the parent plants (2% v/w). Many typical mint volatiles were emitted by the in vitro production, but the call...

We previously demonstrated that sucrose and jasmonic acid (JA) interact duringin photosynthetic in the regulation of development and pigment metab­ olism of the potato (Solanum tuberosum L cv. Sante) grown in vitro. In this study, the research was extended to the effect of light irradiance and quality on sucrose and JA mediated changes. Potato plantlets grown from stem node cul­ ture were grown...

Light quality evaluations were conducted on yam (Dioscorea alata L. cultivar 'Oriental' ) plantlets to determine the potential usefulness of manipulation of the light environment in regulating growth. Eight-week old, in vitro grown yam plantlets were transferred to pots and treated in vivo with end-ofday (EOD) red (R) or far-red (FR) light. Also, nodal segments ofD. alata plantlets were culture...

Cryopreservation has been recognized as a practical and efficient tool for long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of sucrose concentration, hardening temperature and different cryopreservation methods on the survival rate of potato shoot tips after cryopreservation. Excised shoot tips of in vitro plantlets of potato cultivars, At...

This study establishes a protocol for the induction of somatic embryogenesis in Anthurium andraeanum cv. Eidibel. The experiment was arranged in a completely randomized 5 x 5 x 5 factorial design using five explant types (whole leaves, half leaves; petiole; nodal segments and root segments) from in vitro plantlets; five auxins: indole-3-acetic acid (IAA), α-naphthaleneacetic acid (NAA), indole-...

This research work aimed to enhance the concentration of some bioactive compounds in culantro (Eryngium foetidum L.) plantlets through gamma irradiation (0.0, 10.0, 20.0 and 40.0 Gy range) treatments. Gamma irradiation at 40.0 Gy significantly stimulated total phenolic, flavonoids, tannin and saponin contents. The effect of irradiation on antioxidant properties of culantro extracts was investig...