Perception of microbial signal molecules is part of the strategy evolved by plants to survive attacks by potential pathogens. To gain a more complete understanding of the early signaling events involved in these responses, we used fungal components of Fusarium under in vitro condition and checked the rise in signal molecule, salicylic acid (SA), and marker enzymes in defense reactions against t...

DOG(R)1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOG(R)1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l(-1) 2-deoxyglucose (2-DOG) as the ...

A method has been developed for plant regeneration by encapsulation of somatic embryos obtained from callus cultures derived from semi-mature cotyledon explants of Dalbergia sissoo Roxb. (family Fabaceae). Embryogenic callus was developed from cotyledon pieces on Murashige and Skoog (1962) medium supplemented with 9.04 μM 2,4dichlorophenoxyacetic acid and 0.46 μM kinetin. The somatic embryos we...

An efficient in vitro regeneration protocol was established for Swertia chirayita. The present study describes the regeneration of S. chirayita from shoot tip explants collected from wild grown plantlets. The highest number of shoots (26.10 ± 0.73) were obtained on Murashige and Skoog basal medium supplemented with 6-benzyladenine (BA) (0.5mg/l) in combination with gibberellic acid (GA3) (1.0 m...

Gerunggang (Cratoxylum arborescens (Vahl) Blume) is a tree native to Indonesian peatlands that has great potential as an alternative fiber-producing species for the pulp and paper industry because it adaptable does not have disrupt local ecosystems. The also contains important anti-cancer compounds pharmaceutical industry. These industries require large quantities of raw materials meet their ne...

While genotype-environment interaction is increasingly receiving attention by ecologists and evolutionary biologists, such studies need genetically homogeneous replicates-a challenging hurdle in outcrossing plants. This could be potentially overcome by using tissue culture techniques. However, plants regenerated from tissue culture may show aberrant phenotypes and "somaclonal" variation. Here, ...

strawberry is a valuable, nutritious, and economically important fruit all over the world including bangladesh. therefore, there is a demand to develop a suitable variety of strawberry. for this purpose, leaf explants from in vitro grown strawberry plantlets were cultured onto ms medium supplemented with different concentrations and combinations of 2,4-d, naa and ba for callus induction. the mo...

Salinization is a key soil degradation process. An estimated 20% of total cultivated lands and 33% irrigated agricultural worldwide are affected by high salinity. Much research has investigated the influence salt (mainly NaCl) on plants, but very little known about how this related to natural salinity osmotic stress. Therefore, our study was conducted determine ionic stress responses selected C...

To achieve large-scale low-cost ex vitro acclimatization of Siberian ginseng plants, heart- and torpedo-shaped secondary somatic embryos (SEs) induced from germinated SEs on agar medium were collected and then inoculated to 10-l bubble column bioreactor, respectively. For plantlet conversion, inoculation of torpedo-shaped secondary SEs was more effective than heart-shaped SEs. TS2 (culture of t...

Cattleya intermedia is an Atlantic Forest species endemic to Brazil that is classed as vulnerable on the list of threatened species. In this study, C. intermedia plantlets were micropropagated in an asymbiotic culture and the influence of different concentrations of sucrose (15, 30, 45 and 60 g L-1, plus a zero sucrose medium) and macronutrient salts (complete Murashige and Skoog (MS) medium an...