Our purpose is to test if Pluronic® fluorescent nanomicelles can be used for in vivo two-photon imaging of both the normal and the tumor vasculature. The nanomicelles were obtained after encapsulating a hydrophobic two-photon dye: di-stryl benzene derivative, in Pluronic block copolymers. Their performance with respect to imaging depth, blood plasma staining, and diffusion across the tumor vasc...

abstract        background and objective: measurement of amino acids is an important tool for metabolic studies and evaluation of patients’ clinical condition. the aim of this study was to analyze the plasma amino acids using reverse phase high performance liquid chromatography techniques (rp-hplc) with pre-column derivatization by o-phthalaldehyde (opa) in combination with 3- mercaptopropionic...

LLC-PK1 cells have been stably transfected with cDNAs encoding the human norepinephrine transporter (NET), rat dopamine transporter (DAT), and rat serotonin transporter. Using these cell lines, the specificity of each transporter toward agents that inhibit substrate influx and stimulate substrate efflux across the plasma membrane was examined. With 1-methyl-4-phenylpyridinium as a substrate for...

The semisynthetic antibiotic roxithromycin (RXM) exists in an (E)-configuration. Metabolites of RXM in the bile of four cholecystectomy patients with T-tube drainage and in the urine and plasma of four healthy volunteers after single oral doses of 150 mg of RXM were investigated. A total of 15 metabolites were found in bile, urine, and plasma by HPLC with ion trap mass spectrometric and electro...

Gaussian scale space permits one to compute image derivatives. The limitation to some finite order is not inherent in the paradigm itself (Gaussian blurred functions are always smooth), but is caused by the interplay of (at least) two external factors. One is the ratio of the Gaussian scale parameter versus the atomic scale that limits physically or perceptually meaningful sizes (e.g. pixel siz...