A total of 480 nasal samples from apparently healthy Nigerian students were collected aseptically and analyzed bacteriologically. Staphylococci recovered 432 (90%) the subjects, constituting 288 (66.7%) 144 (33.3%) S. aureus epidermidis respectively. The in-vitro antibiotic susceptibility testing using disc diffusion technique showed high multiple resistance to most commonly used antibiotics by...

Fecal samples were collected from diarrheic camel-calves owned by nomadic pastoralists in the Butana Region. They were examined for 5 types of bacteria reported to cause the diarrhea. Sixty nine E. coli (66%), 14 Salmonella spp. (13%) and 11 Clostridium perfringens (10%) isolates were obtained from the samples. However, neither Campylobacter spp. nor Yersinia enterocolitiaca were isolated. The ...

BACKGROUND Electroporation is an established technique for enhancing plasmid delivery to many tissues in vivo, including the skin. We have previously demonstrated efficient delivery of plasmid DNA to the skin utilizing a custom-built four-plate electrode. The experiments described here further evaluate cutaneous plasmid delivery using in vivo electroporation. Plasmid expression levels are compa...

Colistin resistance in Acinetobacter spp. is increasing, resulting potentially untreatable nosocomial infections. Plasmid-mediated colistin of particular concern due to its low fitness cost and potential transferability other bacterial strains species. This study investigated the mechanism a clinical nosocomialis isolate from Cape Town, South Africa. A colistin-resistant A. was identified blood...

Nonviral gene therapy is expected to become a regular treatment for a variety of difficult-to-treat diseases, such as cancer and virus infection. Plasmid DNA, which is used in most nonviral gene delivery systems, usually contains, unmethylated cytosine-guanine dinucleotides, so called CpG motifs. CpG motifs are recognized by immune cells as a danger signal, leading to an inflammatory response. ...

Objective: The aim of the present study is to evaluate the plasmid profile and curing in extended spectrum beta-lactamases (ESBL) producers and know the association of biofilm formation among ESBLs. Methods: Standard disk diffusion method (Kirby Bauer’s method) was performed to know the sensitivity and resistance patterns for commonly used antibiotics and clinical laboratory standard institute ...

Vaccine-elicited cytotoxic T lymphocytes (CTL) should be long-lived memory cells that can rapidly expand in number following re-exposure to antigen. The present studies were initiated to analyze the ability of plasmid interleukin-12 (IL-12) to augment CTL responses in mice when delivered during the peak phase of an immune response elicited by a plasmid human immunodeficiency virus type 1 gp120 ...