The remarkable stability of peptide nucleic acids (PNAs) towards enzymatic degradation makes this class of molecules ideal to develop as part of a diagnostic device. Here we report the development of chemically engineered PNAs for the quantitative detection of HIV RNA at clinically relevant levels that are competitive with current PCR-based assays. Using a sandwich hybridization approach, chemi...

Peptide nucleic acid (PNA) is one of the most widely used synthetic DNA analogs. Conjugation of functional molecules to PNA is very effective to further widen its potential applications. For this purpose, here we report the synthesis of several ligand monomers and introduced them to PNA. These ligand-modified PNAs attract cerium ion and are useful for site-selective DNA hydrolysis. It should be...

Peptide nucleic acids (PNA) are mimics with normal bases connected to a pseudopeptide chain that obey Watson-Crick rules to form stable duplexes with itself and natural nucleic acids. This has focused attention on PNA as therapeutic or diagnostic reagents. Duplexes formed with PNA mirror some but not all properties of DNA. One fascinating aspect of PNA biochemistry is their reaction with enzyme...

Peptide nucleic acid (PNA) is known to bind with extraordinarily high affinity and sequence-specificity to complementary nucleic acid sequences and can be used to suppress gene expression. However, effective delivery into cells is a major obstacle to the development of PNA for gene therapy applications. Here, we present a novel method for the in vitro delivery of antigene PNA to cells. By using...

It was demonstrated that the reaction of some cell species to peanut agglutinin (PNA) involved the maturity of the cells. We investigated the selective adhesion of circulating hemopoietic cells and lymphoid cells to hepatic sinusoidal cells. We experimented with thymocyte (TYC) models that could be easily isolated into PNA positive [PNA(+)] and PNA negative [PNA(-)] subsets, and transfused thes...

PURPOSE Direct sequencing (DS) is the standard method for detection of epidermal growth factor receptor (EGFR) gene mutation in non-small cell lung cancer (NSCLC); however, low detection sensitivity is a problem. The aim of this study is to demonstrate higher detection rate of EGFR gene mutation with peptide nucleic acid (PNA) clamping compared with DS. MATERIALS AND METHODS This is a single ...

The Pacific–North American (PNA) teleconnection is a major mode of Northern Hemisphere wintertime climate variability, with well-known impacts on North American temperature and precipitation. To assess whether the PNA teleconnection has extended predictability, comprehensive data analysis is conducted to elucidate PNA evolution, with an emphasis on patterns of PNA development and decay. These p...