In recent years, mitomycin C (Fig. 1) has been widely used for cancer treatment, but its clinical applications are limited owing to inherent severe toxicity. The therapeutic efficacy of mitomycin C is closely related to the drug concentration in blood and tissues, depending on the dose, route and frequency of administration. Quantitation of mitomycin C in biological fluids was first attained by...

Polarographic technique has been used to evaluate the kinetic parameters and formation constants of Co (П) complexes with benzyl penicillin sodium salt at pH 6.0±0.10 and constant ionic strength (μ = 0.1), HCl in aqueous, aqueous-methanol and aqueous-ethanol mixtures at d.m.e. The effect of concentration and temperature on the half wave potential of the complexes has also been investigated. The...

Inherited disturbances of the mitochondrial energy generating system represent a heterogeneous group of disorders associated with a broad spectrum of metabolic abnormalities and clinical symptoms. We used the polarographic and spectrophotometric method for detection of mitochondrial disorders, because these two techniques provide a different insight into mitochondrial function. In six patients ...

A novel oxygen sensor which does not rely on electrochemical reduction has been used to measure the oxygenation of the murine sarcoma F in a comparative study with an existing polarographic electrode that is available commercially. The prototype luminescence sensor yielded an oxygen distribution comparable with readings made using a pO2 histograph. The percentage of regions detected that had a ...

Normal inclividuals breathing pure oxygen obtain full saturation of their hemoglobin, whereas those with right to left shunts do not. Oxygen hemoglobin dissociation curves indicate that increases to full oxygen saturation of hemoglobin are accompanied by marked increases of the oxygen tension. WVhen breathing oxygen fails to produce full saturation, relatively little change in oxygen tension oc...

PURPOSE We provide a new procedure to quantify in situ corneal oxygen uptake using the micropolarographic Clark electrode. METHODS Traditionally, upon placing a membrane-covered Clark microelectrode onto a human cornea, the resulting polarographic signal is interpreted as the oxygen partial pressure at the anterior corneal surface. However, the Clark electrode operates at a limiting current. ...

the polarographic reduction of aromatic carbodiimides in anhydrous aprotic solvents goes through a carbene intermediate by a diffusion controlled process in two one-electron steps. the first step is the rate-determining step and converts the carbodiirnide to a radical anion (6) that reduces further by a fast one-electron step to a carbene dianion intermediate (7). this abstracts an oxygen, alon...