Cyanate is undergoing study as a drug to prevent occlusive sickle cell crises. Carbamylation of hemoglobin S increases its oxygen affinity, thereby decreasing its tendency to aggregate at low oxygen tensions. The cell membrane has also been shown to be carbamylated. We studied the effect of carbamylation on deformability of sickle, normal, and stored normal erythrocytes. Deformability was measu...

Abstract A new method for isolation of methane oxidising bacteria (methanotrophs) is presented. Soil samples from a wetland area and a landfill were plated on polycarbonate membranes, which were incubated in a methane-air atmosphere using a non-sterile soil suspension as the medium. The membrane acted as a permeable growth support. The membrane method resulted in selective growth conditions, wh...

Methods. Chemotaxis of endothelial cells toward vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) was examined using a modified Boyden chamber assay. Endothelial cells placed in the upper chamber were allowed to migrate through a polycarbonate membrane with 8 pm pores toward VEGF or bFGF present in the lower chamber. Next, the ability of TIMP-3 to inhibit chemo...

Single wall carbon nanotubes (SWCNTs) dispersed by negatively charged sodium deoxycholate (DOC) or positively cetrimonium bromide (CTAB) are shown to assemble into aligned films (3.8 cm2) on polycarbonate membranes slow flow dead-end filtration. Global alignment (S2D max ? 0.85) is obtained both pristine polyvinylpyrrolidone (PVP) coated and those with an intentional 150–600 nm groove pattern f...