نتایج جستجو برای: polymerase chain reaction rt
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مقدمه: candidiasis منتشره، دیابت، حاملگی، مصرف بی رویه ی آنتی بیوتیک های وسیع الطیف، عوامل مستعد کننده ی candidiasis دستگاه ادراری می باشند. شیوع عفونت با گونه های غیر candida albicans رو به افزایش است. هدف از انجام این مطالعه، بررسی میزان شیوع گونه های candida مسبب کاندیدوری در شهر اصفهان با استفاده از روش polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) بود. روش ه...
conclusions this study showed that cccdna can be detected in the plasma specimen of 17.9% of iranian treatment-naive patients with chronic hepatitis b infection. therefore, designing prospective studies focusing on the detection of cccdna in these patients would provide more information. results the mean (sd) age of the patients was 41.1 ± 12.4 years (range, 20 - 62 years). from a total of 106 ...
escherichia coli o157:h7 is an important human pathogen causing haemorrhagic colitis, haemolyticuraemic syndrom and thrombotic thrombocytopenic purpura. in this study, 100 ground beef samples were collected randomly from beef markets in june 2004. for isolation of the bacteria, samples were firstly enriched in modified trypticase soy broth, followed by plating onto sorbitol macconkey agar suppl...
results our study showed that there were no statistically significant differences in the allele and genotype frequencies of -1661 a/g snp between patients with sle and healthy controls (p > 0.05). conclusions in conclusion, we found no association between snp-1661 a/g of the ctla-4 promoter region and susceptibility to sle in our population. objectives to evaluate the potential influences of co...
today, it has been confirmed that the viral infections play an important role in premature delivery and abortion. this study was carried out to determine the frequency rate of papilloma virus types 16 and 18 in pregnant women and their relationship with premature delivery and abortion. in this study, vaginal secretions were collected from pregnant women who had been referred to the women clinic...
تغییرات بیان ژن mmp-2 و mmp-9 در سلول های بنیادی مزانشیمی مغز استخوان انسانی تحت تاثیر فاکتورهای رشد
از آن جا که سلولهای بنیادی مزانشیمی(mesenchymal stem cell,msc) توانایی تبدیل شدن به سلولهایی از رده های مختلف را در شرایط آزمایشگاه نشان داده اند. جداسازی و تکثیر به نسبت راحت و اتولوگ بودن سلولهای مزانشیم آنها را کاندید مناسب برای سلول درمانی ساخته است.اکثر مطالعات آزمایشگاهی نشان دادند که سلول های بنیادی مزانشیمی توانایی گریز از سیستم ایمنی را دارند وپاسخ های ایمنی را نیز مهار می کنند که هر د...
A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (RT-nPCR) procedure that identifies the North American strain of WN virus directly in equine and av...
Abortion in dairy cattle is generally defined as the loss of fetus on days 47-265 of pregnancy and can significantly reduce the income and capital gain of farmers and producers. Therefore, sufficient economical support and preventive measurements are essential for this issue. In this study, genetic materials were extracted from the abomasum rennet, as well as homogenous preparations of brain, k...
Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...
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