background and aims: the aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcdna3.1(+) in bsr cell line. this construct might be used for a potential dna vaccine. materials and methods: glycoprotein gene was synthesized and cloned into pbluescript vector and then sub cloned into eukaryotic expression vector (pcdna3.1(+)). after verifica...

objective: toxoplasmosis can lead to severe pathological effects in both infected humans and animals. the various dna vaccines against toxoplasma compose of single or cocktail antigens have been investigated but they have partial protective against disease. in this study, we used pcrop1 as a dna vaccine and aluminium phosphate and aluminium hydroxide to compare their efficacy as mineral adjuvan...

Although the use of recombinant hepatitis B virus surface (HBsAg) protein vaccine has successfully reduced global hepatitis B infection, there are still a number of vaccine recipients who do not develop detectable antibody responses. Various novel vaccination approaches, including DNA vaccines, have been used to further improve the coverage of vaccine protection. Our previous studies demonstrat...

backgrounds: most of the hepatitis c virus (hcv) infections elicit poor immune responses and 75% to 85% of cases become chronic therefore, the development of an effective vaccine against hcv is of paramount importance. in this study, we aimed to evaluate co-administration of hcv non-structural protein 2 and il-12 dna vaccines in c57bl/6 mice. methods: a plasmid encoding full-length hcv ns2 prot...

Objective(s): Vaccination is one of the most effective means to protect humans and animals against brucellosis. Live attenuated Brucella vaccines are considered effective in animals but they may be potentially infectious to humans, so it is vital to improve the immunoprotective effects and safety of vaccines against Brucella. This study was designed to evaluate the immunogenicity of DNA vaccine...

background: dna immunization with plasmid dna encoding bacterial, viral, parasitic, and tumor antigens has been reported to trigger protective immunity. the use of plasmid dna vaccinations against many diseases has produced promising results in animal and human clinical trials; however, safety concerns about the use of dna vaccines exist, such as the possibility of integration into the host gen...

toxoplasmosis is a worldwide infection which is commonly asymptomatic but can cause serious medical problems in immunocompromised individuals and fetus. the infection also causes considerable economic loss because of abortion in livestock, mostly in sheep and goats. dna vaccination may be a powerful approach against intracellular parasites such as toxoplasma gondii. the goal of this study was t...

conclusions the results of this study demonstrated that the tpasp-padre-truncated orf2 gene cassette and the truncated orf2 gene in recombinant plasmids are successfully expressed in eukaryotic cells. the immunogenicity of the two recombinant plasmids with different formulations will be evaluated as a novel dna vaccine in future investigations. results sequence analysis and bioinformatics studi...

We consider the semigroup $S$ of highest weights appearing in tensor powers $V^{otimes k}$ of a finite dimensional representation $V$ of a connected reductive group. We describe the cone generated by $S$ as the cone over the weight polytope of $V$ intersected with the positive Weyl chamber. From this we get a description for the asymptotic of the number of highest weights appearing in $V^{otime...