The rates at which biological interactions occur can provide important information on the mechanism and behavior of such processes in living systems. This paper will discuss how affinity chromatography can be used as a tool to examine the kinetics of biological interactions. This approach, referred to here as biointeraction chromatography, uses a column with an immobilized binding agent to exam...

1. Gray CH, Urn CK, Nicholson DC. The differentiation of the porphyrins by means of high pressure liquid chromatography. Clin Chim Acta 77, 167-178 (1977). 2. Doss M. Analytical and preparative thinlayer chromatography of porphyrin methyl esters. Z Kiln Chem Kiln Biochem 8, 197207 (1970). 3. Straka JG, Kushner, JP, Burnham BF. High-performance liquid chromatography of porphyrin esters. Identifi...

CLINICAL CHEMISTRY, Vol. 26, No. 6, 1980 789 interference with analysis for theophylline by high-performance liquid chromatography. Clin. Chem. 24, 1847 (1978). Letter. 4. Kelly, R. C. Prentice, D. E., and Hearne, G. M., Cephalosporin antibiotics interfere with the analysis for theophylline by highpressure liquid chromatography. Clin. Chem. 24, 838 (1978). Letter. 5. Orcutt, J. S., Kozak, P. P....

A simple and efficient method, ionic liquid-based dispersive liquid-liquid microextraction combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV), has been applied for the extraction and determination of some antioxidants (Irganox 1010, Irganox 1076 and Irgafos 168) in water samples. The microextraction efficiency factors were investigated and optimized: 1-hexyl-3-...

The coupling of gas and liquid chromatography with mass spectrometry is addressed from the historical point of view. This paper discusses the more important coupling interfaces developed for GC/MS and LC/MS, including the moving belt interface, direct liquid introduction interfaces, thermospray and atmospheric pressure ionization interfaces such as atmospheric pressure chemical ionization and e...

A method was developed for the simultaneous determination of urinary vanilmandelic acid and homovanillic acid, which included a two step prepurification and a reversed-phase high-performance liquid chromatography with amperometric detection. Conditions were evaluated for performing measurements with the amperometric detector free from electric interference. The method was linear between 2.5 and...

The increased separation efficiency provided by the new technology of column packed with core-shell particles in high-performance liquid chromatography (HPLC) has resulted in their widespread diffusion in several analytical fields: from pharmaceutical, biological, environmental, and toxicological. The present paper presents their most recent applications in food analysis. Their use has proved t...

We have developed a simplified and improved high performance liquid chromatography (HPLC) method for the detection and quantitation of tissue lipid using a new laser light-scattering detector (Varex model ELSD II). This detector has a limit of sensitivity of 50 ng for neutral lipid and 200 ng for most phospholipids with excellent reproducibility. By coupling the ELSD II with a ternary gradient ...