Drought is one of the most detrimental environmental stressors to plants with potential decrease crop yields and affect agricultural sustainability. Native bacteria beneficial traits enhance plant growth help avoid reverse effects drought in a greater extent. In present study, we aimed ( i ) isolate drought-tolerant Bacillus isolates from rhizosphere soil wheat grown at different locations Jais...

welsh onion (allium fistulosum l.) is one of the important vegetable crops in china. however, the seeds of this crop are short-lived and their vitality tends to be easily lost in the course of storage. the present study was conducted to elucidate the effects of welsh onion seed priming with potassium nitrate (kno3) on seed vigor and on other such related mechanisms during twelve months of stora...

Seed priming is a technique which is potentially able to promote rapid and more uniform seed germination and plant growth as well. In this regards, the presowing effects of seed priming treatments were investigated on some physiological viz. seed germination, germination rate, radicle elongation, shoot elongation, radicle dry weight, shoot dry weight and vigor index. Seeds were subjected to two...

Intact wild-type tomato (Lycopersicon esculentum cv. Moneymaker) seeds do not complete germination to the same percentage or at the same speed as intact ABA-deficient sitiens (sit") mutant seeds when seeds of both genotypes are imbibed on polyethylene glycol (PEG) solutions of -0 .3 to —1.5 MPa osmotic potential. However, if the thicker testas of wild-type seeds are removed (stripped) from the ...

Four methods of extraction and three methods of concentration of three enteric viruses from mussels were comparatively evaluated by reverse transcriptase PCR (RT-PCR). Shellfish were experimentally contaminated by immersion in seawater seeded with astrovirus, hepatitis A virus, or poliovirus. Sixty-gram samples of mussel tissues were processed by using borate buffer, glycine solution, saline be...

The Leuconostoc species require sucrose in the culture medium as an inducer for the elaboration of glucosyltransferase (GTF) and glucans. Among all the reported putification methods, polyethylene glycol (PEG) is a effective and single step purification method for GTF from Leuconostoc mesenteroides. The extracellular GTFs from Leuconostoc mesenteroides L.m.28 and mutant Leuconostoc mesenteroides...

Cytoplasm contains a large number of macromolecules at extremely high densities. How this striking nature of intracellular milieu called macromolecular crowding affects intracellular signaling remains uncharacterized. Here, we examined the effect of macromolecular crowding on ERK MAPK phosphorylation by MEK MAPKK. Addition of polyethylene glycol-6000 (PEG-6000) as a crowder to mimic intracellul...

Use of cytokines as biomarkers for disease is getting more widespread. Cytokines are conveniently determined by immunoassay, but interference from present antibodies is known to cause problems. In rheumatoid arthritis (RA), interference of rheumatoid factor (RF) may be problematic. RF covers a group of autoantibodies from immunoglobulin subclasses and is present in 65-80% of RA patients. Partly...

We evaluated six precipitation methods for high-density lipoprotein cholesterol (HDL-chol) determination: the heparin/Mn2+ precipitation reagent method (Hep), two variants of the phosphotungstic acid/Mg2+ method (Tung-L and Tung-B), the dextran sulfate 50,000/Mg2+ method (Dex), the PEG 6000 method (PEG), and the PEG 6000/dextran sulfate 15,000 (PEG/Dex) method. The Tung-B and PEG/Dex precipitat...

Aluminium (Al) toxicity and drought are the two major abiotic stress factors limiting common bean production in the tropics. Using hydroponics, the short-term effects of combined Al toxicity and drought stress on root growth and Al uptake into the root apex were investigated. In the presence of Al stress, PEG 6000 (polyethylene glycol)-induced osmotic (drought) stress led to the amelioration of...